Titre
Localized in vivo 13C NMR spectroscopy of the brain.
Type
synthèse (review)
Institution
Externe
Périodique
Auteur(s)
Gruetter, R.
Auteure/Auteur
Adriany, G.
Auteure/Auteur
Choi, I.Y.
Auteure/Auteur
Henry, P.G.
Auteure/Auteur
Lei, H.
Auteure/Auteur
Oz, G.
Auteure/Auteur
Liens vers les personnes
ISSN
0952-3480
Statut éditorial
Publié
Date de publication
2003
Volume
16
Numéro
6-7
Première page
313
Dernière page/numéro d’article
338
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S. ; ReviewPublication Status: ppublish
Résumé
Localized (13)C NMR spectroscopy provides a new investigative tool for studying cerebral metabolism. The application of (13)C NMR spectroscopy to living intact humans and animals presents the investigator with a number of unique challenges. This review provides in the first part a tutorial insight into the ingredients required for achieving a successful implementation of localized (13)C NMR spectroscopy. The difficulties in establishing (13)C NMR are the need for decoupling of the one-bond (13)C-(1)H heteronuclear J coupling, the large chemical shift range, the low sensitivity and the need for localization of the signals. The methodological consequences of these technical problems are discussed, particularly with respect to (a) RF front-end considerations, (b) localization methods, (c) the low sensitivity, and (d) quantification methods. Lastly, some achievements of in vivo localized (13)C NMR spectroscopy of the brain are reviewed, such as: (a) the measurement of brain glutamine synthesis and the feasibility of quantifying glutamatergic action in the brain; (b) the demonstration of significant anaplerotic fluxes in the brain; (c) the demonstration of a highly regulated malate-aspartate shuttle in brain energy metabolism and isotope flux; (d) quantification of neuronal and glial energy metabolism; and (e) brain glycogen metabolism in hypoglycemia in rats and humans. We conclude that the unique and novel insights provided by (13)C NMR spectroscopy have opened many new research areas that are likely to improve the understanding of brain carbohydrate metabolism in health and disease.
PID Serval
serval:BIB_4225356D09EF
PMID
Open Access
Oui
Date de création
2010-08-04T14:28:12.589Z
Date de création dans IRIS
2025-05-20T19:47:10Z