Titre
Scaf1 promotes respiratory supercomplexes and metabolic efficiency in zebrafish.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
García-Poyatos, C.
Auteure/Auteur
Cogliati, S.
Auteure/Auteur
Calvo, E.
Auteure/Auteur
Hernansanz-Agustín, P.
Auteure/Auteur
Lagarrigue, S.
Auteure/Auteur
Magni, R.
Auteure/Auteur
Botos, M.
Auteure/Auteur
Langa, X.
Auteure/Auteur
Amati, F.
Auteure/Auteur
Vázquez, J.
Auteure/Auteur
Mercader, N.
Auteure/Auteur
Enríquez, J.A.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1469-3178
Statut éditorial
Publié
Date de publication
2020-07-03
Volume
21
Numéro
7
Première page
e50287
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
The oxidative phosphorylation (OXPHOS) system is a dynamic system in which the respiratory complexes coexist with super-assembled quaternary structures called supercomplexes (SCs). The physiological role of SCs is still disputed. Here, we used zebrafish to study the relevance of respiratory SCs. We combined immunodetection analysis and deep data-independent proteomics to characterize these structures and found similar SCs to those described in mice, as well as novel SCs including III <sub>2</sub> + IV <sub>2</sub> , I + IV, and I + III <sub>2 </sub> + IV <sub>2</sub> . To study the physiological role of SCs, we generated two null allele zebrafish lines for supercomplex assembly factor 1 (scaf1). scaf1 <sup>-/-</sup> fish displayed altered OXPHOS activity due to the disrupted interaction of complexes III and IV. scaf1 <sup>-/-</sup> fish were smaller in size and showed abnormal fat deposition and decreased female fertility. These physiological phenotypes were rescued by doubling the food supply, which correlated with improved bioenergetics and alterations in the metabolic gene expression program. These results reveal that SC assembly by Scaf1 modulates OXPHOS efficiency and allows the optimization of metabolic resources.
PID Serval
serval:BIB_05E1F4028C2C
PMID
Open Access
Oui
Date de création
2020-06-04T20:37:55.167Z
Date de création dans IRIS
2025-05-20T18:32:25Z
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Nom
Garcia_Poyatos_EMBOreports_2020.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by/4.0
Taille
2.7 MB
Format
Adobe PDF
PID Serval
serval:BIB_05E1F4028C2C.P001
URN
urn:nbn:ch:serval-BIB_05E1F4028C2C9
Somme de contrôle
(MD5):23a8d02efe2b8b304c2ced5ab1a906c9