Titre
An abundant tissue macrophage population in the adult murine heart with a distinct alternatively-activated macrophage profile.
Type
article
Institution
Externe
Périodique
Auteur(s)
Pinto, A.R.
Auteure/Auteur
Paolicelli, R.
Auteure/Auteur
Salimova, E.
Auteure/Auteur
Gospocic, J.
Auteure/Auteur
Slonimsky, E.
Auteure/Auteur
Bilbao-Cortes, D.
Auteure/Auteur
Godwin, J.W.
Auteure/Auteur
Rosenthal, N.A.
Auteure/Auteur
Liens vers les personnes
ISSN
1932-6203
Statut éditorial
Publié
Date de publication
2012
Volume
7
Numéro
5
Première page
e36814
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
Cardiac tissue macrophages (cTMs) are a previously uncharacterised cell type that we have identified and characterise here as an abundant GFP(+) population within the adult Cx(3)cr1(GFP/+) knock-in mouse heart. They comprise the predominant myeloid cell population in the myocardium, and are found throughout myocardial interstitial spaces interacting directly with capillary endothelial cells and cardiomyocytes. Flow cytometry-based immunophenotyping shows that cTMs exhibit canonical macrophage markers. Gene expression analysis shows that cTMs (CD45(+)CD11b(+)GFP(+)) are distinct from mononuclear CD45(+)CD11b(+)GFP(+) cells sorted from the spleen and brain of adult Cx(3)cr1(GFP/+) mice. Gene expression profiling reveals that cTMs closely resemble alternatively-activated anti-inflammatory M2 macrophages, expressing a number of M2 markers, including Mrc1, CD163, and Lyve-1. While cTMs perform normal tissue macrophage homeostatic functions, they also exhibit a distinct phenotype, involving secretion of salutary factors (including IGF-1) and immune modulation. In summary, the characterisation of cTMs at the cellular and molecular level defines a potentially important role for these cells in cardiac homeostasis.
Sujets
PID Serval
serval:BIB_8F442D30F14F
PMID
Open Access
Oui
Date de création
2018-12-18T09:44:26.336Z
Date de création dans IRIS
2025-05-20T23:44:05Z
Fichier(s)![Vignette d'image]()
En cours de chargement...
Nom
22590615_BIB_8F442D30F14F.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by/4.0
Taille
4.36 MB
Format
Adobe PDF
PID Serval
serval:BIB_8F442D30F14F.P001
Somme de contrôle
(MD5):23db3082878885052381a1fca71db7f0