Titre
Evaluation of Sézary cell marker expression and cell death behaviour upon in vitro treatment by flow cytometry in Sézary syndrome patients.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Melchers, S.
Auteure/Auteur
Roemer, M.
Auteure/Auteur
Albrecht, J.D.
Auteure/Auteur
Assaf, C.
Auteure/Auteur
von Gugelberg, C.
Auteure/Auteur
Guenova, E.
Auteure/Auteur
Klemke, C.D.
Auteure/Auteur
Moritz, RKC
Auteure/Auteur
Schlaak, M.
Auteure/Auteur
Stadler, R.
Auteure/Auteur
Wehkamp, U.
Auteure/Auteur
Wobser, M.
Auteure/Auteur
Albrecht, T.
Auteure/Auteur
Goerdt, S.
Auteure/Auteur
Schneider, S.
Auteure/Auteur
Nicolay, J.P.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1600-0625
Statut éditorial
Publié
Date de publication
2024-09
Volume
33
Numéro
9
Première page
e15171
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
The diagnosis of Sézary syndrome (SS) relies on the identification of blood Sézary cells (SC) by different markers via flow cytometry. Treatment of SS is challenging since its pathogenesis is characterized by cell death resistance rather than hyperproliferation. In this study, we establish an integrated approach that considers both the expression of SC markers and sensitivity to cell death both spontaneously and upon in vitro treatment. Peripheral blood mononuclear cells were isolated from 20 SS patients and analysed for the SC markers CD7 and CD26 loss as well as CD158k and PD1 gain. The cells were then treated with different established and experimental therapies in vitro and cell death was measured. Spontaneous and therapeutically induced cell death were measured and correlated to cellular marker profiles. In the marker-positive cells, spontaneous cell death sensitivity was reduced. Different treatments in vitro managed to specifically induce cell death in the putative CTCL cell populations. Interestingly, a repeated analysis after 3 months of treatment revealed the CTCL cell death sensitivity to be restored by therapy. We propose this novel integrated approach comprising the evaluation of SC marker expression and analysis of cell death sensitivity upon treatment that can also enable a better therapy stratification.
Sujets
PID Serval
serval:BIB_0D74764CCDB9
PMID
Open Access
Oui
Date de création
2024-09-09T12:50:45.453Z
Date de création dans IRIS
2025-05-20T13:28:45Z
Fichier(s)![Vignette d'image]()
En cours de chargement...
Nom
39219147.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by/4.0
Taille
928.64 KB
Format
Adobe PDF
PID Serval
serval:BIB_0D74764CCDB9.P001
URN
urn:nbn:ch:serval-BIB_0D74764CCDB98
Somme de contrôle
(MD5):7bcc1f77319aeb62ceb53ff9a49bf557