Titre
Encapsulation of peptides in biodegradable microspheres prolongs their MHC class-I presentation by dendritic cells and macrophages in vitro
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Audran, R.
Auteure/Auteur
Peter, K.
Auteure/Auteur
Dannull, J.
Auteure/Auteur
Men, Y.
Auteure/Auteur
Scandella, E.
Auteure/Auteur
Groettrup, M.
Auteure/Auteur
Gander, B.
Auteure/Auteur
Corradin, G.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0264-410X
Statut éditorial
Publié
Date de publication
2003-03
Volume
21
Numéro
11-12
Première page
1250
Dernière page/numéro d’article
5
Peer-reviewed
Oui
Langue
anglais
Notes
Comparative Study
Evaluation Studies
Journal Article --- Old month value: Mar 7
Evaluation Studies
Journal Article --- Old month value: Mar 7
Résumé
Biodegradable microspheres (MS) consisting of poly(D,L-lactide-co-glycolide) (PLGA) represent a promising alternative to conventional adjuvants. The adjustable pulsatile release of encapsulated material from such MS offers the potential to mimic the priming and boosting injections of conventional immunization regimens. In this paper, we demonstrate that MS can serve as antigen reservoirs in antigen presenting cells (APC), so that antigen is presented for extended periods of time (up to 9 days). In particular, we could show by measurement of IFN-gamma production that encapsulated peptides were presented to cytotoxic T lymphocytes (CTL) by mouse and human macrophages as well as by human dendritic cells in vitro for a longer time period as compared to soluble peptides. The extended antigen presentation may thus improve the CTL response in vivo. These results may be of paramount importance in cancer vaccination therapy since MS may serve as antigen reservoirs to extend the presentation time by APC used to boost the patient's immune response to tumor antigens.
Sujets
PID Serval
serval:BIB_EF0B0D971D04
PMID
Date de création
2008-01-24T13:55:41.408Z
Date de création dans IRIS
2025-05-21T05:14:37Z