Titre
N2-succinylornithine in ornithine catabolism of Pseudomonas aeruginosa.
Type
article
Institution
Externe
Périodique
Auteur(s)
Vander Wauven, C.
Auteure/Auteur
Jann, A.
Auteure/Auteur
Haas, D.
Auteure/Auteur
Leisinger, T.
Auteure/Auteur
Stalon, V.
Auteure/Auteur
Liens vers les personnes
ISSN
0302-8933
Statut éditorial
Publié
Date de publication
1988
Volume
150
Numéro
4
Première page
400
Dernière page/numéro d’article
404
Langue
anglais
Résumé
Most Pseudomonas aeruginosa PAO mutants which were unable to utilize L-arginine as the sole carbon and nitrogen source (aru mutants) under aerobic conditions were also affected in L-ornithine utilization. These aru mutants were impaired in one or several enzymes involved in the conversion of N2-succinylornithine to glutamate and succinate, indicating that the latter steps of the arginine succinyltransferase pathway can be used for ornithine catabolism. Addition of aminooxyacetate, an inhibitor of the N2-succinylornithine 5-aminotransferase, to resting cells of P. aeruginosa in ornithine medium led to the accumulation of N2-succinylornithine. In crude extracts of P. aeruginosa an ornithine succinyltransferase (L-ornithine:succinyl-CoA N2-succinyltransferase) activity could be detected. An aru mutant having reduced arginine succinyltransferase activity also had correspondingly low levels of ornithine succinyltransferase. Thus, in P. aeruginosa, these two activities might be due to the same enzyme, which initiates aerobic arginine and ornithine catabolism.
PID Serval
serval:BIB_8AA08F1B6055
PMID
Date de création
2008-01-25T16:01:07.000Z
Date de création dans IRIS
2025-05-20T23:04:40Z