Titre
Cholinergic neurons of fetal rat telencephalon in aggregating cell culture respond to NGF as well as to protein kinase C-activating tumor promoters.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Developmental Brain Research
Auteur(s)
Honegger, P.
Auteure/Auteur
Du Pasquier, P.
Auteure/Auteur
Tenot, M.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0165-3806
Statut éditorial
Publié
Date de publication
1986
Volume
394
Numéro
2
Première page
217
Dernière page/numéro d’article
223
Langue
anglais
Résumé
Serum-free aggregating cell cultures of fetal rat telencephalon treated with the potent tumor promoter phorbol 12-myristate 13-acetate (PMA) showed a dose-dependent, persistent stimulation of the enzymes choline acetyltransferase (ChAT), glutamic acid decarboxylase and glutamine synthetase. After elimination of the proliferating cells by treatment of the cultures with Ara-C (0.4 microM) only the cholinergic marker enzyme, ChAT, could be stimulated by tumor promoters. The non-promoting phorbol ester, 4 alpha-phorbol 12,13-didecanoate proved to be inactive in these cultures, whereas the potent non-phorbol tumor promoter, mezerein, produced an even greater stimulatory effect than PMA. Since PMA and mezerein are potent and specific activators of protein kinase C, the present results suggest a role for this second messenger in the development of cholinergic telencephalon neurons. Stimulation of ChAT required prolonged exposure (48 h) of the cultures to PMA and the responsiveness of the cholinergic neurons to the tumor promoters decreased with progressive cellular maturation. The cholinergic telencephalon neurons showed the same pattern of responsiveness for tumor promoters as for nerve growth factor (NGF). However, the combined treatment with NGF and either PMA or mezerein produced an additive stimulatory effect, suggesting somewhat different mechanisms of action.
PID Serval
serval:BIB_1E2A6F158EEC
PMID
Date de création
2008-01-24T12:12:01.070Z
Date de création dans IRIS
2025-05-20T19:11:26Z