Titre
Polyunsaturated fatty acid deficiency reverses effects of alcohol on mitochondrial energy metabolism
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Piquet, M. A.
Auteure/Auteur
Roulet, M.
Auteure/Auteur
Nogueira, V.
Auteure/Auteur
Filippi, C.
Auteure/Auteur
Sibille, B.
Auteure/Auteur
Hourmand-Ollivier, I.
Auteure/Auteur
Pilet, M.
Auteure/Auteur
Rouleau, V.
Auteure/Auteur
Leverve, X. M.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0168-8278
Statut éditorial
Publié
Date de publication
2004-11
Volume
41
Numéro
5
Première page
721
Dernière page/numéro d’article
9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Research Support, Non-U.S. Gov't --- Old month value: Nov
Résumé
BACKGROUND/AIMS: Polyunsaturated fatty acids (PUFA) deficiency is common in patients with alcoholic liver disease. The suitability of reversing such deficiency remains controversial. The aim was to investigate the role played by PUFA deficiency in the occurrence of alcohol-related mitochondrial dysfunction. METHODS: Wistar rats were fed either a control diet with or without alcohol (control and ethanol groups) or a PUFA deficient diet with or without alcohol (PUFA deficient and PUFA deficient+ethanol groups). After 6 weeks, liver mitochondria were isolated for energetic studies and fatty acid analysis. RESULTS: Mitochondria from ethanol fed rats showed a dramatic decrease in oxygen consumption rates and in cytochrome oxidase activity. PUFA deficiency showed an opposite picture. PUFA deficient+ethanol group roughly reach control values, regarding cytochrome oxidase activity and respiratory rates. The relationship between ATP synthesis and respiratory rate was shifted to the left in ethanol group and to the right in PUFA-deficient group. The plots of control and PUFA deficient+ethanol groups were overlapping. Phospholipid arachidonic over linoleic ratio closely correlated to cytochrome oxidase and oxygen uptake. CONCLUSIONS: PUFA deficiency reverses alcohol-related mitochondrial dysfunction via an increase in phospholipid arachidonic over linoleic ratio, which raises cytochrome oxidase activity. Such deficiency may be an adaptive mechanism.
Sujets
PID Serval
serval:BIB_E78A923A562C
PMID
Date de création
2008-01-24T19:33:14.186Z
Date de création dans IRIS
2025-05-21T05:30:42Z