Titre
Glucocorticoid regulation of mouse mammary tumor virus: identification of a short essential DNA region
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Buetti, E.
Auteure/Auteur
Diggelmann, H.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0261-4189
Statut éditorial
Publié
Date de publication
1983
Volume
2
Numéro
8
Première page
1423
Dernière page/numéro d’article
1429
Notes
Journal Article Research Support, Non-U.S. Gov't
Résumé
Transcription of mouse mammary tumor virus (MMTV) DNA is stimulated by steroid hormones. To determine the DNA sequences involved in this regulation, we constructed a plasmid containing the MMTV long terminal repeat (LTR) in front of the coding region of the herpes simplex thymidine kinase gene, from which the promoter had been removed. Portions of the LTR were removed by the nuclease Ba/31, and the deleted molecules were recloned and tested for transcriptional activity in transfections of Ltk-aprt- cells. Stably transfected cell clones were selected and hormone-dependent transcription from the MMTV promoter was studied by the S1 nuclease mapping method. The results show that DNA sequences between -105 and -204 base pairs upstream from the initiation site of viral transcription are required for glucocorticoid stimulation.
Sujets
PID Serval
serval:BIB_AB53C749C865
PMID
Date de création
2008-01-25T13:25:22.608Z
Date de création dans IRIS
2025-05-20T21:40:48Z
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Nom
BIB_AB53C749C865.P001.pdf
Version du manuscrit
preprint
Taille
1.84 MB
Format
Adobe PDF
PID Serval
serval:BIB_AB53C749C865.P001
URN
urn:nbn:ch:serval-BIB_AB53C749C8652
Somme de contrôle
(MD5):f5e05154277d28b93541cf0c57f60586