Titre
Correlative light and electron microscopy using immunolabeled resin sections.
Type
chapitre
Institution
Externe
Auteur(s)
Schwarz, H.
Auteure/Auteur
Humbel, B.M.
Auteure/Auteur
Liens vers les personnes
Maison dāĆ©dition
Springer
Titre du livre ou confƩrence/colloque
Methods in Molecular Biology
Statut Ć©ditorial
PubliƩ
Date de publication
2007
Volume
369
Chapitre
12
PremiĆØre page
229
DerniĆØre page/numĆ©ro dāarticle
256
Langue
anglais
RƩsumƩ
In correlative microscopy, light microscopy provides the overview and orientation in the complex cells and tissue, whereas electron microscopy offers the detailed localization and correlation to subcellular structures. In this chapter, we offer the detailed high-quality electron microscopical preparation methods for the optimum preservation of the cellular ultrastructure. From such preparations, serial thin sections are collected and used for comparative histochemical, immunofluorescence, and immunogold staining. In light microscopy, histological stains are used to identify the orientation of the sample, and immunofluorescence labeling is used to identify the region of interest, namely, the labeled cells expressing the macromolecule under investigation. Subsequent sections, labeled with immunogold, are analyzed by electron microscopy to identify the label within the cellular architecture at high resolution.
Sujets
PID Serval
serval:BIB_38FC9978AF7D
PMID
Date de crƩation
2012-02-28T17:43:56.180Z
Date de crƩation dans IRIS
2025-05-20T18:19:00Z