Titre
Systematic analysis of differential rhythmic liver gene expression mediated by the circadian clock and feeding rhythms.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Auteur(s)
Weger, B.D.
Auteure/Auteur
Gobet, C.
Auteure/Auteur
David, FPA
Auteure/Auteur
Atger, F.
Auteure/Auteur
Martin, E.
Auteure/Auteur
Phillips, N.E.
Auteure/Auteur
Charpagne, A.
Auteure/Auteur
Weger, M.
Auteure/Auteur
Naef, F.
Auteure/Auteur
Gachon, F.
Auteure/Auteur
Liens vers les unités
ISSN
1091-6490
Statut éditorial
Publié
Date de publication
2021-01-19
Volume
118
Numéro
3
Première page
e2015803118
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
The circadian clock and feeding rhythms are both important regulators of rhythmic gene expression in the liver. To further dissect the respective contributions of feeding and the clock, we analyzed differential rhythmicity of liver tissue samples across several conditions. We developed a statistical method tailored to compare rhythmic liver messenger RNA (mRNA) expression in mouse knockout models of multiple clock genes, as well as PARbZip output transcription factors (Hlf/Dbp/Tef). Mice were exposed to ad libitum or night-restricted feeding under regular light-dark cycles. During ad libitum feeding, genetic ablation of the core clock attenuated rhythmic-feeding patterns, which could be restored by the night-restricted feeding regimen. High-amplitude mRNA expression rhythms in wild-type livers were driven by the circadian clock, but rhythmic feeding also contributed to rhythmic gene expression, albeit with significantly lower amplitudes. We observed that Bmal1 and Cry1/2 knockouts differed in their residual rhythmic gene expression. Differences in mean expression levels between wild types and knockouts correlated with rhythmic gene expression in wild type. Surprisingly, in PARbZip knockout mice, the mean expression levels of PARbZip targets were more strongly impacted than their rhythms, potentially due to the rhythmic activity of the D-box-repressor NFIL3. Genes that lost rhythmicity in PARbZip knockouts were identified to be indirect targets. Our findings provide insights into the diurnal transcriptome in mouse liver as we identified the differential contributions of several core clock regulators. In addition, we gained more insights on the specific effects of the feeding-fasting cycle.
Sujets
PID Serval
serval:BIB_E4689F566897
PMID
Open Access
Oui
Date de création
2021-02-15T09:36:08.959Z
Date de création dans IRIS
2025-05-21T05:53:18Z
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Nom
Systematic_e2015803118.full.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by-nc-nd/4.0
Taille
5.57 MB
Format
Adobe PDF
PID Serval
serval:BIB_E4689F566897.P001
URN
urn:nbn:ch:serval-BIB_E4689F5668974
Somme de contrôle
(MD5):d7c164283329276a19ea6f77ea2e41e3