Titre
Identification of five new HLA-B*3501-restricted epitopes derived from common melanoma-associated antigens, spontaneously recognized by tumor-infiltrating lymphocytes.
Type
article
Institution
Externe
Périodique
Auteur(s)
Benlalam, H.
Auteure/Auteur
Linard, B.
Auteure/Auteur
Guilloux, Y.
Auteure/Auteur
Moreau-Aubry, A.
Auteure/Auteur
Derré, L.
Auteure/Auteur
Diez, E.
Auteure/Auteur
Dreno, B.
Auteure/Auteur
Jotereau, F.
Auteure/Auteur
Labarrière, N.
Auteure/Auteur
Liens vers les personnes
ISSN
0022-1767
Statut éditorial
Publié
Date de publication
2003
Volume
171
Numéro
11
Première page
6283
Dernière page/numéro d’article
6289
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
We previously described HLA-B35-restricted melanoma tumor-infiltrating lymphocyte responses to frequently expressed melanoma-associated Ags: tyrosinase, Melan-A/MART-1, gp100, MAGE-A3/MAGE-A6, and NY-ESO-1. Using clones derived from these TIL, we identified in this study the corresponding epitopes. We show that five of these epitopes are new and that melanoma cells naturally present all the six epitopes. Interestingly, five of these epitopes correspond to or encompass melanoma-associated Ag epitopes presented in other HLA contexts, such as A2, A1, B51, and Cw3. In particular, the HLA-B35-restricted Melan-A epitope is mimicked by the peptide 26-35, already known as the most immunodominant melanoma epitope in the HLA-A*0201 context. Because this peptide lacked adequate anchor amino acid residues for efficient binding to HLA-B35, modified peptides were designed. Two of these analogues were found to induce higher PBL- and tumor-infiltrating lymphocyte-specific responses than the parental peptide, suggesting that they could be more immunogenic in HLA-B*3501 melanoma patients. These data have important implications for the formulation of polypeptide-based vaccines as well as for the monitoring of melanoma-specific CTL response in HLA-B*3501 melanoma patients.
Sujets
PID Serval
serval:BIB_B8FF502638B1
PMID
Open Access
Oui
Date de création
2014-03-31T09:03:10.903Z
Date de création dans IRIS
2025-05-20T21:49:39Z
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BIB_B8FF502638B1.P001.pdf
Version du manuscrit
published
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Format
Adobe PDF
PID Serval
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