Titre
A rapid microbiopsy system to improve the preservation of biological samples prior to high-pressure freezing.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Vanhecke, D.
Auteure/Auteur
Graber, W.
Auteure/Auteur
Herrmann, G.
Auteure/Auteur
Al-Amoudi, A.
Auteure/Auteur
Eggli, P.
Auteure/Auteur
Studer, D.
Auteure/Auteur
Liens vers les unités
ISSN
0022-2720
Statut éditorial
Publié
Date de publication
2003
Volume
212
Numéro
Pt 1
Première page
3
Dernière page/numéro d’article
12
Langue
anglais
Notes
Old uritopublisher value http://www.blackwell-synergy.com/Journals/member/institutions/issuelist.asp?journal=jmi
Résumé
A microbiopsy system for fast excision and transfer of biological specimens from donor to high-pressure freezer was developed. With a modified, commercially available, Promag 1.2 biopsy gun, tissue samples can be excised with a size small enough (0.6 mm x 1.2 mm x 0.3 mm) to be easily transferred into a newly designed specimen platelet. A self-made transfer unit allows fast transfer of the specimen from the needle into the specimen platelet. The platelet is then fixed in a commercially available specimen holder of a high-pressure freezing machine (EM PACT, Leica Microsystems, Vienna, Austria) and frozen therein. The time required by a well-instructed (but not experienced) person to execute all steps is in the range of half a minute. This period is considered short enough to maintain the excised tissue pieces close to their native state. We show that a range of animal tissues (liver, brain, kidney and muscle) are well preserved. To prove the quality of freezing achieved with the system, we show vitrified ivy leaves high-pressure frozen in the new specimen platelet.
PID Serval
serval:BIB_28885
PMID
Date de création
2007-11-19T11:26:13.006Z
Date de création dans IRIS
2025-05-20T16:29:25Z