Titre
Cross-reactivity of an antiserum to the alpha-subunit of the Na+, K+-ATPase of toad (Bufo marinus) kidney with basal and apical membranes of transporting epithelia of the rat
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Graves, J. S.
Auteure/Auteur
Inabnett, T.
Auteure/Auteur
Geering, K.
Auteure/Auteur
Simson, J. A.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0302-766X
Statut éditorial
Publié
Date de publication
1989-10
Volume
258
Numéro
1
Première page
137
Dernière page/numéro d’article
45
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Oct
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Oct
Résumé
An antibody to the 96 kD alpha-subunit of the Na+, K+-ATPase from Bufo marinus has been used in immunostaining rat kidney and salivary glands. Intense staining was observed on basolateral membranes of distal tubules of the kidney and striated ducts of the three major salivary glands. Less intense staining was seen on the basolateral membranes of parotid acinar cells, but no staining was seen on the acinar cells of submandibular or sublingual glands. These sites of staining have been shown, by other methods, to posses substantial Na+, K+-ATPase, indicating that the antibody recognizes antigenic determinants of the sodium pump highly conserved in the course of evolution. In addition, staining with this antibody was observed at the apical region of cells of the proximal straight tubule and of the papillary collecting duct in the kidney. Absorption studies suggest that the apical antigenic determinants are the same or closely related to each other but are distinct from basolateral antigenic determinants.
Sujets
PID Serval
serval:BIB_577E4F233ED5
PMID
Date de création
2008-01-24T11:28:38.933Z
Date de création dans IRIS
2025-05-20T14:41:41Z