Titre
Biologically controlled mineralization in the hypercalcified sponge Petrobiona massiliana (Calcarea, Calcaronea)
Type
article
Institution
Externe
Périodique
Auteur(s)
Gilis, M.
Auteure/Auteur
Baronnet, A.
Auteure/Auteur
Dubois, P.
Auteure/Auteur
Legras, L.
Auteure/Auteur
Grauby, O.
Auteure/Auteur
Willenz, P.
Auteure/Auteur
Liens vers les personnes
ISSN
1047-8477
Statut éditorial
Publié
Date de publication
2012
Volume
178
Première page
279
Dernière page/numéro d’article
289
Peer-reviewed
Oui
Langue
anglais
Résumé
Hypercalcified sponges, endowed with a calcium carbonate basal skeleton
in addition to their spicules, form one of the most basal metazoan group
engaged in extensive biomineralization. The Mediterranean species
Petrobiona massiliana was used to investigate biological controls
exerted on the biomineralization of its basal skeleton. Scanning and
transmission electron microscopy (SEM, TEM) confirmed that
basopinacocytes form a discontinuous layer of flattened cells covering
the skeleton and display ultrastructural features attesting intense
secretory activity. The production of a highly structured fibrillar
organic matrix framework by basopinacocytes toward the growing skeleton
was highlighted both by potassium pyroantimonate and ruthenium red
protocols, the latter further suggesting the presence of sulfated
glycosaminoglycans in the matrix. Furthermore organic material
incorporated into the basal skeleton was shown by SEM and TEM at
different structural levels while its response to alcian blue and
acridine orange staining might suggest a similar acidic and sulfated
chemical composition in light microscopy. Potassium pyroantimonate
revealed in TEM and energy electron loss spectroscopy (EELS) analysis,
heavy linear precipitates 100-300 nm wide containing Ca2+ and Mg2+ ions,
either along the basal cell membrane of basopinacocytes located toward
the decalcified basal skeleton or around decalcified spicules in the
mesohyl. Based on the results of the previous mineralogical
characterization and the present work, an hypothetical model of
biomineralization is proposed for P. massiliana: basopinacocytes would
produce an extracellular organic framework that might guide the
assemblage of submicronic amorphous Ca- and Mg-bearing grains into
higher structural units. (c) 2012 Elsevier Inc. All rights reserved.
in addition to their spicules, form one of the most basal metazoan group
engaged in extensive biomineralization. The Mediterranean species
Petrobiona massiliana was used to investigate biological controls
exerted on the biomineralization of its basal skeleton. Scanning and
transmission electron microscopy (SEM, TEM) confirmed that
basopinacocytes form a discontinuous layer of flattened cells covering
the skeleton and display ultrastructural features attesting intense
secretory activity. The production of a highly structured fibrillar
organic matrix framework by basopinacocytes toward the growing skeleton
was highlighted both by potassium pyroantimonate and ruthenium red
protocols, the latter further suggesting the presence of sulfated
glycosaminoglycans in the matrix. Furthermore organic material
incorporated into the basal skeleton was shown by SEM and TEM at
different structural levels while its response to alcian blue and
acridine orange staining might suggest a similar acidic and sulfated
chemical composition in light microscopy. Potassium pyroantimonate
revealed in TEM and energy electron loss spectroscopy (EELS) analysis,
heavy linear precipitates 100-300 nm wide containing Ca2+ and Mg2+ ions,
either along the basal cell membrane of basopinacocytes located toward
the decalcified basal skeleton or around decalcified spicules in the
mesohyl. Based on the results of the previous mineralogical
characterization and the present work, an hypothetical model of
biomineralization is proposed for P. massiliana: basopinacocytes would
produce an extracellular organic framework that might guide the
assemblage of submicronic amorphous Ca- and Mg-bearing grains into
higher structural units. (c) 2012 Elsevier Inc. All rights reserved.
PID Serval
serval:BIB_242035083C60
Date de création
2013-01-03T14:00:37.940Z
Date de création dans IRIS
2025-05-20T20:36:23Z