Titre
Generation of cytotoxic T lymphocytes in vitro. II. Effect of repeated exposure to alloantigens on the cytotoxic activity of long-term mixed leukocyte cultures.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Macdonald, H.R.
Auteure/Auteur
Engers, H.D.
Auteure/Auteur
Cerottini, J.C.
Auteure/Auteur
Brunner, K.T.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0022-1007
Statut éditorial
Publié
Date de publication
1974-09-01
Volume
140
Numéro
3
Première page
718
Dernière page/numéro d’article
730
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Mouse cytotoxic T lymphocytes (CTL) were generated in unidirectional mixed leukocyte cultures (MLC) using normal C57BL/6 spleen cells as responding cells and irradiated DBA/2 spleen cells as stimulating cells. Cytotoxicity was assayed on (51)Cr-labeled P-815 (DBA/2) target cells, and the relative frequency of CTL in individual cell populations was estimated from dose-response curves. Upon inclusion of 2-mercaptoethanol in the culture medium, it was found that significant CTL activity could be detected for as long as 3 wk in primary MLC. Reexposure of MLC cells to the original stimulating alloantigens after 14-41 days in culture resulted in significant cell proliferation and rapid regeneration of high levels of immunologically specific cytotoxicity. CTL activity in these secondary cultures increased dramatically within the first 24 h and reached higher peak levels than those found at the peak of the primary response. Furthermore, proliferation and reappearance of CTL activity could be demonstrated following each of as many as four sequential alloantigenic stimulations of the same initial cell population at 20-day intervals. Interestingly, cells recovered from MLC at the peak of the primary response on day 4 were insensitive to further allogeneic stimulation. Taken together, these results are consistent with the hypothesis that CTL differentiate in MLC to become long-lived memory cells which gradually lose their cytotoxic activity. Upon reexposure to specific alloantigen, such memory CTL rapidly regain their functional activity and proliferate to generate an expanded CTL population.
Sujets
PID Serval
serval:BIB_994194CA3C84
PMID
Open Access
Oui
Date de création
2008-01-28T10:14:46.067Z
Date de création dans IRIS
2025-05-21T00:36:59Z
Fichier(s)![Vignette d'image]()
En cours de chargement...
Nom
4278109_BIB_994194CA3C84.pdf
Version du manuscrit
published
Taille
707.04 KB
Format
Adobe PDF
PID Serval
serval:BIB_994194CA3C84.P001
Somme de contrôle
(MD5):c58e32e3d8d4d8c0c028878090c9f77e