Titre
The Structure of the Mouse Serotonin 5-HT3 Receptor in Lipid Vesicles.
Type
article
Institution
Externe
Périodique
Auteur(s)
Kudryashev, M.
Auteure/Auteur
Castaño-Díez, D.
Auteure/Auteur
Deluz, C.
Auteure/Auteur
Hassaine, G.
Auteure/Auteur
Grasso, L.
Auteure/Auteur
Graf-Meyer, A.
Auteure/Auteur
Vogel, H.
Auteure/Auteur
Stahlberg, H.
Auteure/Auteur
Liens vers les personnes
ISSN
1878-4186
Statut éditorial
Publié
Date de publication
2016-01-05
Volume
24
Numéro
1
Première page
165
Dernière page/numéro d’article
170
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
The function of membrane proteins is best understood if their structure in the lipid membrane is known. Here, we determined the structure of the mouse serotonin 5-HT3 receptor inserted in lipid bilayers to a resolution of 12 Å without stabilizing antibodies by cryo electron tomography and subtomogram averaging. The reconstruction reveals protein secondary structure elements in the transmembrane region, the extracellular pore, and the transmembrane channel pathway, showing an overall similarity to the available X-ray model of the truncated 5-HT3 receptor determined in the presence of a stabilizing nanobody. Structural analysis of the 5-HT3 receptor embedded in a lipid bilayer allowed the position of the membrane to be determined. Interactions between the densely packed receptors in lipids were visualized, revealing that the interactions were maintained by the short horizontal helices. In combination with methodological improvements, our approach enables the structural analysis of membrane proteins in response to voltage and ligand gating.
PID Serval
serval:BIB_807A636DEBA0
PMID
Open Access
Oui
Date de création
2023-06-09T14:02:52.589Z
Date de création dans IRIS
2025-05-21T00:03:20Z