Titre
Combination of Hyaluronan and Lyophilized Progenitor Cell Derivatives: Stabilization of Functional Hydrogel Products for Therapeutic Management of Tendinous Tissue Disorders.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Laurent, A.
Auteure/Auteur
Porcello, A.
Auteure/Auteur
Fernandez, P.G.
Auteure/Auteur
Jeannerat, A.
Auteure/Auteur
Peneveyre, C.
Auteure/Auteur
Abdel-Sayed, P.
Auteure/Auteur
Scaletta, C.
Auteure/Auteur
Hirt-Burri, N.
Auteure/Auteur
Michetti, M.
Auteure/Auteur
de Buys Roessingh, A.
Auteure/Auteur
Raffoul, W.
Auteure/Auteur
Allémann, E.
Auteure/Auteur
Jordan, O.
Auteure/Auteur
Applegate, L.A.
Auteure/Auteur
Liens vers les personnes
ISSN
1999-4923
Statut éditorial
Publié
Date de publication
2021-12-19
Volume
13
Numéro
12
Première page
2196
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
Cultured progenitor cells and derivatives have been used in various homologous applications of cutaneous and musculoskeletal regenerative medicine. Active pharmaceutical ingredients (API) in the form of progenitor cell derivatives such as lysates and lyophilizates were shown to retain function in controlled cellular models of wound repair. On the other hand, hyaluronan-based hydrogels are widely used as functional vehicles in therapeutic products for tendon tissue disorders. The aim of this study was the experimental characterization of formulations containing progenitor tenocyte-derived APIs and hyaluronan, for the assessment of ingredient compatibility and stability in view of eventual therapeutic applications in tendinopathies. Lyophilized APIs were determined to contain relatively low quantities of proteins and growth factors, while being physicochemically stable and possessing significant intrinsic antioxidant properties. Physical and rheological quantifications of the combination formulas were performed after hydrogen peroxide challenge, outlining significantly improved evolutive viscoelasticity values in accelerated degradation settings. Thus, potent effects of physicochemical protection or stability enhancement of hyaluronan by the incorporated APIs were observed. Finally, combination formulas were found to be easily injectable into ex vivo tendon tissues, confirming their compatibility with further translational clinical approaches. Overall, this study provides the technical bases for the development of progenitor tenocyte derivative-based injectable therapeutic products or devices, to potentially be applied in tendinous tissue disorders.
PID Serval
serval:BIB_DBCEF906F288
PMID
Open Access
Oui
Date de création
2022-01-03T14:54:14.835Z
Date de création dans IRIS
2025-05-21T01:00:01Z
Fichier(s)![Vignette d'image]()
En cours de chargement...
Nom
34959477_BIB_DBCEF906F288.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by/4.0
Taille
2.15 MB
Format
Adobe PDF
PID Serval
serval:BIB_DBCEF906F288.P001
URN
urn:nbn:ch:serval-BIB_DBCEF906F2889
Somme de contrôle
(MD5):9a7fc533d89555962851a3e8eb740e6e