Titre
Report from the HarmoSter study: inter-laboratory comparison of LC-MS/MS measurements of corticosterone, 11-deoxycortisol and cortisone.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Fanelli, F.
Auteure/Auteur
Bruce, S.
Auteure/Auteur
Cantù, M.
Auteure/Auteur
Temchenko, A.
Auteure/Auteur
Mezzullo, M.
Auteure/Auteur
Lindner, J.M.
Auteure/Auteur
Peitzsch, M.
Auteure/Auteur
Binz, P.A.
Auteure/Auteur
Ackermans, M.T.
Auteure/Auteur
Heijboer, A.C.
Auteure/Auteur
Van den Ouweland, J.
Auteure/Auteur
Koeppl, D.
Auteure/Auteur
Nardi, E.
Auteure/Auteur
Rauh, M.
Auteure/Auteur
Vogeser, M.
Auteure/Auteur
Eisenhofer, G.
Auteure/Auteur
Pagotto, U.
Auteure/Auteur
Liens vers les unités
ISSN
1437-4331
Statut éditorial
Publié
Date de publication
2023-01-27
Volume
61
Numéro
1
Première page
67
Dernière page/numéro d’article
77
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) panels that include glucocorticoid-related steroids are increasingly used to characterize and diagnose adrenal cortical diseases. Limited information is currently available about reproducibility of these measurements among laboratories. The aim of the study was to compare LC-MS/MS measurements of corticosterone, 11-deoxycortisol and cortisone at eight European centers and assess the performance after unification of calibration.
Seventy-eight patient samples and commercial calibrators were measured twice by laboratory-specific procedures. Results were obtained according to in-house and external calibration. We evaluated intra-laboratory and inter-laboratory imprecision, regression and agreement against performance specifications derived from 11-deoxycortisol biological variation.
Intra-laboratory CVs ranged between 3.3 and 7.7%, 3.3 and 11.8% and 2.7 and 12.8% for corticosterone, 11-deoxycortisol and cortisone, with 1, 4 and 3 laboratories often exceeding the maximum allowable imprecision (MAI), respectively. Median inter-laboratory CVs were 10.0, 10.7 and 6.2%, with 38.5, 50.7 and 2.6% cases exceeding the MAI for corticosterone, 11-deoxycortisol and cortisone, respectively. Median laboratory bias vs. all laboratory-medians ranged from -5.6 to 12.3% for corticosterone, -14.6 to 12.4% for 11-deoxycortisol and -4.0 to 6.5% for cortisone, with few cases exceeding the total allowable error. Modest deviations were found in regression equations among most laboratories. External calibration did not improve 11-deoxycortisol and worsened corticosterone and cortisone inter-laboratory comparability.
Method imprecision was variable. Inter-laboratory performance was reasonably good. However, cases with imprecision and total error above the acceptable limits were apparent for corticosterone and 11-deoxycortisol. Variability did not depend on calibration but apparently on imprecision, accuracy and specificity of individual methods. Tools for improving selectivity and accuracy are required to improve harmonization.
Seventy-eight patient samples and commercial calibrators were measured twice by laboratory-specific procedures. Results were obtained according to in-house and external calibration. We evaluated intra-laboratory and inter-laboratory imprecision, regression and agreement against performance specifications derived from 11-deoxycortisol biological variation.
Intra-laboratory CVs ranged between 3.3 and 7.7%, 3.3 and 11.8% and 2.7 and 12.8% for corticosterone, 11-deoxycortisol and cortisone, with 1, 4 and 3 laboratories often exceeding the maximum allowable imprecision (MAI), respectively. Median inter-laboratory CVs were 10.0, 10.7 and 6.2%, with 38.5, 50.7 and 2.6% cases exceeding the MAI for corticosterone, 11-deoxycortisol and cortisone, respectively. Median laboratory bias vs. all laboratory-medians ranged from -5.6 to 12.3% for corticosterone, -14.6 to 12.4% for 11-deoxycortisol and -4.0 to 6.5% for cortisone, with few cases exceeding the total allowable error. Modest deviations were found in regression equations among most laboratories. External calibration did not improve 11-deoxycortisol and worsened corticosterone and cortisone inter-laboratory comparability.
Method imprecision was variable. Inter-laboratory performance was reasonably good. However, cases with imprecision and total error above the acceptable limits were apparent for corticosterone and 11-deoxycortisol. Variability did not depend on calibration but apparently on imprecision, accuracy and specificity of individual methods. Tools for improving selectivity and accuracy are required to improve harmonization.
PID Serval
serval:BIB_2C4CB5F7C0A8
PMID
Open Access
Oui
Date de création
2022-11-02T07:20:07.184Z
Date de création dans IRIS
2025-05-20T13:58:16Z
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Nom
36288389.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by/4.0
Taille
953.86 KB
Format
Adobe PDF
PID Serval
serval:BIB_2C4CB5F7C0A8.P001
URN
urn:nbn:ch:serval-BIB_2C4CB5F7C0A86
Somme de contrôle
(MD5):74eb30930bebed148d9ad38dde43529c