Titre
Simultaneous HPLC analysis of the hypnotic benzodiazepines nitrazepam, estazolam, flunitrazepam, and triazolam in plasma.
Type
article
Institution
Externe
Périodique
Auteur(s)
Boukhabza, A.
Auteure/Auteur
Lugnier, A.A.
Auteure/Auteur
Kintz, P.
Auteure/Auteur
Mangin, P.
Auteure/Auteur
Liens vers les personnes
ISSN
0146-4760[print], 0146-4760[linking]
Statut éditorial
Publié
Date de publication
1991
Volume
15
Numéro
6
Première page
319
Dernière page/numéro d’article
322
Langue
anglais
Résumé
A new analytical methodology was developed for simultaneous high-performance liquid chromatographic quantitation of nitrazepam, estazolam, flunitrazepam, and triazolam in plasma. After addition of the internal standard nor-prazepam, biological samples and calibration standards were extracted at basic pH into diethyl ether-methylene chloride (2:1, v/v). The reconstituted extract was separated on a reversed-phase Nova Pak C18 column with acidic buffer (6mM)-acetonitrile-methanol (64:23:13, v/v) as mobile phase. The detection was performed at 242 nm. Within-run and day-to-day precision values were about 2-8%. The method is thus sensitive and reproducible for use in clinical and experimental toxicokinetic studies.
PID Serval
serval:BIB_DCEC17E65323
PMID
Date de création
2010-10-15T14:18:27.320Z
Date de création dans IRIS
2025-05-21T01:01:21Z