Titre
Cytomegalovirus (CMV) DNA Quantitation in Bronchoalveolar Lavage Fluid From Hematopoietic Stem Cell Transplant Recipients With CMV Pneumonia
Type
article
Institution
Externe
Périodique
Auteur(s)
Boeckh, M.
Auteure/Auteur
Stevens-Ayers, T.
Auteure/Auteur
Travi, G.
Auteure/Auteur
Huang, M. L.
Auteure/Auteur
Cheng, G. S.
Auteure/Auteur
Xie, H.
Auteure/Auteur
Leisenring, W.
Auteure/Auteur
Erard, V.
Auteure/Auteur
Seo, S.
Auteure/Auteur
Kimball, L.
Auteure/Auteur
Corey, L.
Auteure/Auteur
Pergam, S. A.
Auteure/Auteur
Jerome, K. R.
Auteure/Auteur
Liens vers les personnes
ISSN
1537-6613
Statut éditorial
Publié
Date de publication
2017
Volume
215
Numéro
10
Première page
1514
Dernière page/numéro d’article
1522
Langue
anglais
Notes
Boeckh, Michael
Stevens-Ayers, Terry
Travi, Giovanna
Huang, Meei-Li
Cheng, Guang-Shing
Xie, Hu
Leisenring, Wendy
Erard, Veronique
Seo, Sachiko
Kimball, Louise
Corey, Lawrence
Pergam, Steven A
Jerome, Keith R
eng
K24 HL093294/HL/NHLBI NIH HHS/
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
J Infect Dis. 2017 May 15;215(10):1514-1522. doi: 10.1093/infdis/jix048.
Stevens-Ayers, Terry
Travi, Giovanna
Huang, Meei-Li
Cheng, Guang-Shing
Xie, Hu
Leisenring, Wendy
Erard, Veronique
Seo, Sachiko
Kimball, Louise
Corey, Lawrence
Pergam, Steven A
Jerome, Keith R
eng
K24 HL093294/HL/NHLBI NIH HHS/
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
J Infect Dis. 2017 May 15;215(10):1514-1522. doi: 10.1093/infdis/jix048.
Résumé
Background: Quantitative cytomegalovirus (CMV) DNA-specific polymerase chain reaction (PCR) analysis is widely used as a surveillance method for hematopoietic stem cell transplant (HCT) recipients. However, no CMV DNA threshold exists in bronchoalveolar lavage (BAL) to differentiate pneumonia from pulmonary shedding. Methods: We tested archived BAL fluid samples from 132 HCT recipients with CMV pneumonia and 139 controls (100 patients with non-CMV pneumonia, 18 with idiopathic pneumonia syndrome [IPS], and 21 who were asymptomatic) by quantitative CMV and beta-globin DNA-specific PCR. Results: Patients with CMV pneumonia had higher median viral loads (3.9 log10 IU/mL; interquartile range [IQR], 2.6-6.0 log10 IU/mL) than controls (0 log10 IU/mL [IQR, 0-1.6 log10 IU/mL] for patients with non-CMV pneumonia, 0 log10 IU/mL [IQR, 0-1.6 log10 IU/mL] for patients with IPS, and 1.63 log10 IU/mL [IQR, 0-2.5 log10 IU/mL] for patients who were asymptomatic; P < .001 for all comparisons to patients with CMV pneumonia). Receiver operating characteristic curve analyses and predictive models identified a cutoff CMV DNA level of 500 IU/mL to differentiate between CMV pneumonia and pulmonary shedding, using current CMV pneumonia prevalence figures. However, different levels may be appropriate in settings of very high or low CMV pneumonia prevalence. The presence of pulmonary copathogens, radiographic presentation, or pulmonary hemorrhage did not alter predictive values. Conclusion: CMV DNA load in BAL can be used to differentiate CMV pneumonia from pulmonary shedding.
PID Serval
serval:BIB_CD378EBDBE01
PMID
Date de création
2022-02-25T14:43:35.380Z
Date de création dans IRIS
2025-05-21T00:55:40Z