Titre
Multi-phosphorylation reaction and clustering tune Pom1 gradient mid-cell levels according to cell size.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Gerganova, V.
Auteure/Auteur
Floderer, C.
Auteure/Auteur
Archetti, A.
Auteure/Auteur
Michon, L.
Auteure/Auteur
Carlini, L.
Auteure/Auteur
Reichler, T.
Auteure/Auteur
Manley, S.
Auteure/Auteur
Martin, S.G.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
2050-084X
Statut éditorial
Publié
Date de publication
2019-05-03
Volume
8
Première page
e45983
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Publication Status: epublish
Résumé
Protein concentration gradients pattern developing organisms and single cells. In Schizosaccharomyces pombe rod-shaped cells, Pom1 kinase forms gradients with maxima at cell poles. Pom1 controls the timing of mitotic entry by inhibiting Cdr2, which forms stable membrane-associated nodes at mid-cell. Pom1 gradients rely on membrane association regulated by a phosphorylation-dephosphorylation cycle and lateral diffusion modulated by clustering. Using quantitative PALM imaging, we find individual Pom1 molecules bind the membrane too transiently to diffuse from pole to mid-cell. Instead, we propose they exchange within longer lived clusters forming the functional gradient unit. An allelic series blocking auto-phosphorylation shows that multi-phosphorylation shapes and buffers the gradient to control mid-cell levels, which represent the critical Cdr2-regulating pool. TIRF imaging of this cortical pool demonstrates more Pom1 overlaps with Cdr2 in short than long cells, consistent with Pom1 inhibition of Cdr2 decreasing with cell growth. Thus, the gradients modulate Pom1 mid-cell levels according to cell size.
Sujets
PID Serval
serval:BIB_01CBB053B8AE
PMID
Open Access
Oui
Date de création
2019-05-06T15:01:16.082Z
Date de création dans IRIS
2025-05-20T17:58:51Z
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Nom
elife-45983-v2.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by/4.0
Taille
3.44 MB
Format
Adobe PDF
PID Serval
serval:BIB_01CBB053B8AE.P001
URN
urn:nbn:ch:serval-BIB_01CBB053B8AE0
Somme de contrôle
(MD5):4699ab4402f4879b433a1790c7fff350