Titre
Molecular beacon sequence analysis for detecting drug resistance in Mycobacterium tuberculosis
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Piatek, A. S.
Auteure/Auteur
Tyagi, S.
Auteure/Auteur
Pol, A. C.
Auteure/Auteur
Telenti, A.
Auteure/Auteur
Miller, L. P.
Auteure/Auteur
Kramer, F. R.
Auteure/Auteur
Alland, D.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1087-0156
Statut éditorial
Publié
Date de publication
1998-04
Volume
16
Numéro
4
Première page
359
Dernière page/numéro d’article
63
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr
Résumé
We developed a new approach to DNA sequence analysis that uses fluorogenic reporter molecules--molecular beacons--and demonstrated their ability to discriminate alleles in real-time PCR assays of genomic DNA. A set of overlapping molecular beacons was used to analyze an 81-bp region of the Mycobacterium tuberculosis rpoB gene for mutations that confer resistance to the antibiotic rifampin. In a blinded study of 52 rifampin-resistant and 23 rifampin-susceptible clinical isolates, this method correctly detected mutations in all of the resistant strains and in none of the susceptible strains. The assay was carried out entirely in sealed PCR tubes and was simple to perform and interpret. This approach can be used to analyze any DNA sequence of moderate length with single base pair accuracy.
Sujets
PID Serval
serval:BIB_7485BF2BBB9E
PMID
Date de création
2008-01-25T13:45:47.873Z
Date de création dans IRIS
2025-05-20T23:24:41Z