Titre
Ultrastructural evidence of exosome secretion by progenitor cells in adult mouse myocardium and adult human cardiospheres.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Barile, L.
Auteure/Auteur
Gherghiceanu, M.
Auteure/Auteur
Popescu, L.M.
Auteure/Auteur
Moccetti, T.
Auteure/Auteur
Vassalli, G.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1110-7251
Statut éditorial
Publié
Date de publication
2012
Volume
2012
Première page
354605
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article WOS Document Type: Review
Résumé
The demonstration of beneficial effects of cell therapy despite the persistence of only few transplanted cells in vivo suggests secreted factors may be the active component of this treatment. This so-called paracrine hypothesis is supported by observations that culture media conditioned by progenitor cells contain growth factors that mediate proangiogenic and cytoprotective effects. Cardiac progenitor cells in semi-suspension culture form spherical clusters (cardiospheres) that deliver paracrine signals to neighboring cells. A key component of paracrine secretion is exosomes, membrane vesicles that are stored intracellularly in endosomal compartments and are secreted when these structures fuse with the cell plasma membrane. Exosomes have been identified as the active component of proangiogenic effects of bone marrow CD34(+) stem cells in mice and the regenerative effects of embryonic mesenchymal stem cells in infarcted hearts in pigs and mice. Here, we provide electron microscopic evidence of exosome secretion by progenitor cells in mouse myocardium and human cardiospheres. Exosomes are emerging as an attractive vector of paracrine signals delivered by progenitor cells. They can be stored as an "off-the-shelf" product. As such, exosomes have the potential for circumventing many of the limitations of viable cells for therapeutic applications in regenerative medicine.
PID Serval
serval:BIB_E6BB61D40B8C
PMID
Open Access
Oui
Date de création
2012-12-20T17:57:51.748Z
Date de création dans IRIS
2025-05-21T03:08:51Z
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Nom
BIB_E6BB61D40B8C.P001.pdf
Version du manuscrit
preprint
Taille
3.15 MB
Format
Adobe PDF
PID Serval
serval:BIB_E6BB61D40B8C.P001
URN
urn:nbn:ch:serval-BIB_E6BB61D40B8C7
Somme de contrôle
(MD5):f1c2cd86675db935896aec1fef93472d