Titre
Vasopressin-dependent coupling between sodium transport and water flow in a mouse cortical collecting duct cell line.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Gaeggeler, H.P.
Auteure/Auteur
Guillod, Y.
Auteure/Auteur
Loffing-Cueni, D.
Auteure/Auteur
Loffing, J.
Auteure/Auteur
Rossier, B.C.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1523-1755
Statut éditorial
Publié
Date de publication
2011
Volume
79
Numéro
8
Première page
843
Dernière page/numéro d’article
852
Langue
anglais
Résumé
Water balance is achieved through the ability of the kidney to control water reabsorption in the connecting tubule and the collecting duct. In a mouse cortical collecting duct cell line (mCCD(c11)), physiological concentrations of arginine vasopressin increased both electrogenic, amiloride-sensitive, epithelial sodium channel (ENaC)-mediated sodium transport measured by the short-circuit current (Isc) method and water flow (Jv apical to basal) measured by gravimetry with similar activation coefficient K(1/2) (6 and 12 pM, respectively). Jv increased linearly according to the osmotic gradient across the monolayer. A small but highly significant Jv was also measured under isoosmotic conditions. To test the coupling between sodium reabsorption and water flow, mCCD(c11) cells were treated for 24 h under isoosmotic condition with either diluent, amiloride, vasopressin or vasopressin and amiloride. Isc, Jv, and net chemical sodium fluxes were measured across the same monolayers. Around 30% of baseline and 50% of vasopressin-induced water flow is coupled to an amiloride-sensitive, ENaC-mediated, electrogenic sodium transport, whereas the remaining flow is coupled to an amiloride-insensitive, nonelectrogenic sodium transport mediated by an unknown electroneutral transporter. The mCCD(c11) cell line is a first example of a mammalian tight epithelium allowing quantitative study of the coupling between sodium and water transport. Our data are consistent with the 'near isoosmotic' fluid transport model.
PID Serval
serval:BIB_5F5434B8C4D3
PMID
Open Access
Oui
Date de création
2011-04-26T13:13:11.265Z
Date de création dans IRIS
2025-05-20T20:26:20Z