Titre
Efficient T cell activation requires an optimal dwell-time of interaction between the TCR and the pMHC complex.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Kalergis, A.M.
Auteure/Auteur
Boucheron, N.
Auteure/Auteur
Doucey, M.A.
Auteure/Auteur
Palmieri, E.
Auteure/Auteur
Goyarts, E.C.
Auteure/Auteur
Vegh, Z.
Auteure/Auteur
Luescher, I.F.
Auteure/Auteur
Nathenson, S.G.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1529-2908
Statut éditorial
Publié
Date de publication
2001
Volume
2
Numéro
3
Première page
229
Dernière page/numéro d’article
234
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Publication Status: ppublish
Résumé
Cytotoxic T cell (CTL) activation by antigen requires the specific detection of peptide-major histocompatibility class I (pMHC) molecules on the target-cell surface by the T cell receptor (TCR). We examined the effect of mutations in the antigen-binding site of a Kb-restricted TCR on T cell activation, antigen binding and dissociation from antigen.These parameters were also examined for variants derived from a Kd-restricted peptide that was recognized by a CTL clone. Using these two independent systems, we show that T cell activation can be impaired by mutations that either decrease or increase the binding half-life of the TCR-pMHC interaction. Our data indicate that efficient T cell activation occurs within an optimal dwell-time range of TCR-pMHC interaction. This restricted dwell-time range is consistent with the exclusion of either extremely low or high affinity T cells from the expanded population during immune responses.
Sujets
PID Serval
serval:BIB_890D638689DC
PMID
Date de création
2008-01-28T10:20:04.894Z
Date de création dans IRIS
2025-05-21T04:12:17Z