Titre
A reporter virus particle seroneutralization assay for tick-borne encephalitis virus overcomes ELISA limitations.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Ackermann-Gäumann, R.
Auteure/Auteur
Dentand, A.
Auteure/Auteur
Lienhard, R.
Auteure/Auteur
Saeed, M.
Auteure/Auteur
Speiser, D.E.
Auteure/Auteur
MacDonald, M.R.
Auteure/Auteur
Coste, A.T.
Auteure/Auteur
Cagno, V.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1096-9071
Statut éditorial
Publié
Date de publication
2024-08
Volume
96
Numéro
8
Première page
e29843
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Tick-borne encephalitis (TBE) virus is the most prevalent tick-transmitted orthoflavivirus in Europe. Due to the nonspecific nature of its symptoms, TBE is primarily diagnosed by ELISA-based detection of specific antibodies in the patient serum. However, cross-reactivity between orthoflaviviruses complicates the diagnosis. Specificity issues may be mitigated by serum neutralization assays (SNT), although the handling of clinically relevant orthoflaviviruses requires biosafety level (BSL) 3 conditions and they have highly divergent viral kinetics and cell tropisms. In the present study, we established a reporter virus particle (RVP)-based SNT in which the infectivity is measured by luminescence and that can be performed under BSL-2 conditions. The RVP-based SNT for TBEV exhibited a highly significant correlation with the traditional virus-based SNT (R <sup>2</sup> = 0.8637, p < 0.0001). The RVP-based assay demonstrated a sensitivity of 92.3% (95% CI: 79.7%-97.4%) and specificity of 100% (95% CI: 81.6%-100%). We also tested the cross-reactivity of serum samples in RVP-based assays against other orthoflaviviruses (yellow fever virus, dengue virus type 2, Zika virus, West Nile virus and Japanese encephalitis virus). Interestingly, all serum samples which had tested TBEV-positive by ELISA but negative by RVP-based SNT were reactive for antibodies against other orthoflaviviruses. Thus, the RVP-based seroneutralization assay provides an added value in clinical diagnostics as well as in epidemiological studies.
Sujets
PID Serval
serval:BIB_60C8B876F1D2
PMID
Open Access
Oui
Date de création
2024-08-09T14:20:27.467Z
Date de création dans IRIS
2025-05-20T17:45:19Z