Titre
Structural and molecular characterization of astrocyte and vasculature connectivity in the mouse hippocampus and cortex.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Lorin, C.
Auteure/Auteur
Guiet, R.
Auteure/Auteur
Chiaruttini, N.
Auteure/Auteur
Ambrosini, G.
Auteure/Auteur
Boci, E.
Auteure/Auteur
Abdellah, M.
Auteure/Auteur
Markram, H.
Auteure/Auteur
Keller, D.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1098-1136
Statut éditorial
Publié
Date de publication
2024-11
Volume
72
Numéro
11
Première page
2001
Dernière page/numéro d’article
2021
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
The relation of astrocytic endfeet to the vasculature plays a key functional role in the neuro-glia-vasculature unit. We characterize the spatial organization of astrocytes and the structural aspects that facilitate their involvement in molecular exchanges. Using double transgenic mice, we performed co-immunostaining, confocal microscopy, and three-dimensional digital segmentation to investigate the biophysical and molecular organization of astrocytes and their intricate endfoot network at the micrometer level in the isocortex and hippocampus. The results showed that hippocampal astrocytes had smaller territories, reduced endfoot dimensions, and fewer contacts with blood vessels compared with those in the isocortex. Additionally, we found that both connexins 43 and 30 have a higher density in the endfoot and the former is overexpressed relative to the latter. However, due to the limitations of the method, further studies are needed to determine the exact localization on the endfoot. The quantitative information obtained in this study will be useful for modeling the interactions of astrocytes with the vasculature.
Sujets
PID Serval
serval:BIB_7C608FE96D05
PMID
Open Access
Oui
Date de création
2024-07-19T12:57:17.761Z
Date de création dans IRIS
2025-05-21T04:18:33Z
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Nom
39007459.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by/4.0
Taille
4.21 MB
Format
Adobe PDF
PID Serval
serval:BIB_7C608FE96D05.P001
URN
urn:nbn:ch:serval-BIB_7C608FE96D055
Somme de contrôle
(MD5):29a41b9e5bed2ca324a98f1ef65c9db7