Azithromycin alters spatial and temporal dynamics of airway microbiota in idiopathic pulmonary fibrosis.

von Garnier, C.

doi:10.1183/23120541.00720-2022

Titre

Azithromycin alters spatial and temporal dynamics of airway microbiota in idiopathic pulmonary fibrosis.

Type

article

Institution

UNIL/CHUV/Unisanté + institutions partenaires

Périodique

ERJ Open Research

Auteur(s)

Gijs, P.J.

Auteure/Auteur

Daccord, C.

Auteure/Auteur

Bernasconi, E.

Auteure/Auteur

Brutsche, M.

Auteure/Auteur

Clarenbach, C.F.

Auteure/Auteur

Hostettler, K.

Auteure/Auteur

Guler, S.A.

Auteure/Auteur

Mercier, L.

Auteure/Auteur

Ubags, N.

Auteure/Auteur

Funke-Chambour, M.

Auteure/Auteur

von Garnier, C.

Auteure/Auteur

Liens vers les personnes

Von Garnier, Christophe

Daccord, Cécile

Ubags, Niki

Gijs, Pieter-Jan

Liens vers les unités

Pneumologie

ISSN

2312-0541

Statut éditorial

Publié

Date de publication

2023-05

Volume

9

Numéro

3

Première page

00720

Dernière page/numéro d’article

2022

Peer-reviewed

Oui

Langue

anglais

Notes

Publication types: Journal Article
Publication Status: epublish

Résumé

High bacterial burden in the lung microbiota predicts progression of idiopathic pulmonary fibrosis (IPF). Azithromycin (AZT) is a macrolide antibiotic known to alter the lung microbiota in several chronic pulmonary diseases, and observational studies have shown a positive effect of AZT on mortality and hospitalisation rate in IPF. However, the effect of AZT on the lung microbiota in IPF remains unknown.
We sought to determine the impact of a 3-month course of AZT on the lung microbiota in IPF. We assessed sputum and oropharyngeal swab specimens from 24 adults with IPF included in a randomised controlled crossover trial of oral AZT 500 mg 3 times per week. 16S rRNA gene amplicon sequencing and quantitative PCR (qPCR) were performed to assess bacterial communities. Antibiotic resistance genes (ARGs) were assessed using real-time qPCR.
AZT significantly decreased community diversity with a stronger and more persistent effect in the lower airways (sputum). AZT treatment altered the temporal kinetics of the upper (oropharyngeal swab) and lower airway microbiota, increasing community similarity between the two sites for 1 month after macrolide cessation. Patients with an increase in ARG carriage had lower bacterial density and enrichment of the genus Streptococcus. In contrast, patients with more stable ARG carriage had higher bacterial density and enrichment in Prevotella.
AZT caused sustained changes in the diversity and composition of the upper and lower airway microbiota in IPF, with effects on the temporal and spatial dynamics between the two sites.

PID Serval

serval:BIB_A193A919A5E2

DOI

10.1183/23120541.00720-2022

PMID

37228285

WOS

000989622600023

Permalien

https://iris.unil.ch/handle/iris/158675

Open Access

Oui

Date de création

2023-06-05T13:51:32.976Z

Date de création dans IRIS

2025-05-20T23:08:11Z

Fichier(s)

Nom

Article.pdf

Version du manuscrit

preprint

Licence

https://creativecommons.org/licenses/by/4.0

Taille

1.41 MB

Format

Adobe PDF

PID Serval

serval:BIB_A193A919A5E2.P001

URN

urn:nbn:ch:serval-BIB_A193A919A5E20

Somme de contrôle

(MD5):deabb4586295724f8f4979964496cbc4

Nom

Imprimatur.pdf

Version du manuscrit

preprint

Taille

69.05 KB

Format

Adobe PDF

PID Serval

serval:BIB_A193A919A5E2.S001

Somme de contrôle

(MD5):14ce6d619f9e468c8ce0627156482a30