Titre
Somatically acquired recombinant murine leukemia proviruses in thymic leukemias of AKR/J mice.
Type
article
Institution
Externe
Périodique
Auteur(s)
Herr, W.
Auteure/Auteur
Gilbert, W.
Auteure/Auteur
Liens vers les personnes
ISSN
0022-538X
Statut éditorial
Publié
Date de publication
1983
Volume
46
Numéro
1
Première page
70
Dernière page/numéro d’article
82
Langue
anglais
Résumé
We have probed the structure and arrangement of murine leukemia virus genomes in eight spontaneous AKR thymic leukemias by Southern hybridization with one ecotropic pol and four ecotropic env probes. These probes revealed many (in 2 cases over 15) somatically acquired proviruses that had undergone complex patterns of recombination. The large majority were not deleted and were structurally analogous to the oncogenic mink cell focus-inducing murine leukemia viruses isolated from AKR tumors in that the amino-terminal p15E-coding region derived from ecotropic AKR murine leukemia virus sequences, whereas certain gp70-coding sequences were nonecotropic. Nevertheless, we observed a few proviruses which did not appear to be gp70 recombinants; however, these proviruses were in general clearly recombinant within the p15E-coding sequences. Although the proviral recombination patterns were quite variable, in general the large majority of recombinant proviruses within each tumor appeared structurally identical, indicating that they originate from a common parent. Each tumor contained a unique pattern of provirus integrations; densitometer tracings of the Southern hybridizations indicated that many of the integrated proviruses were present at one copy per cell, suggesting that the tumors derive from a single cell which contained multiple integrated copies of a unique recombinant virus structurally similar to the mink cell focus-inducing viruses.
PID Serval
serval:BIB_785B85555508
PMID
Date de création
2008-01-24T14:36:37.662Z
Date de création dans IRIS
2025-05-21T03:13:39Z