Titre
Therapeutic drug monitoring of seven psychotropic drugs and four metabolites in human plasma by HPLC-MS.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Auteur(s)
Choong, E.
Auteure/Auteur
Rudaz, S.
Auteure/Auteur
Kottelat, A.
Auteure/Auteur
Guillarme, D.
Auteure/Auteur
Veuthey, J.L.
Auteure/Auteur
Eap, C.B.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1873-264X[electronic]
Statut éditorial
Publié
Date de publication
2009
Volume
50
Numéro
5
Première page
1000
Dernière page/numéro d’article
1008
Peer-reviewed
Oui
Langue
anglais
Résumé
A simple and sensitive LC-MS method was developed and validated for the simultaneous quantification of aripiprazole (ARI), atomoxetine (ATO), duloxetine (DUL), clozapine (CLO), olanzapine (OLA), sertindole (STN), venlafaxine (VEN) and their active metabolites dehydroaripiprazole (DARI), norclozapine (NCLO), dehydrosertindole (DSTN) and O-desmethylvenlafaxine (OVEN) in human plasma. The above mentioned compounds and the internal standard (remoxipride) were extracted from 0.5 mL plasma by solid-phase extraction (mix mode support). The analytical separation was carried out on a reverse phase liquid chromatography at basic pH (pH 8.1) in gradient mode. All analytes were monitored by MS detection in the single ion monitoring mode and the method was validated covering the corresponding therapeutic range: 2-200 ng/mL for DUL, OLA, and STN, 4-200 ng/mL for DSTN, 5-1000 ng/mL for ARI, DARI and finally 2-1000 ng/mL for ATO, CLO, NCLO, VEN, OVEN. For all investigated compounds, good performance in terms of recoveries, selectivity, stability, repeatability, intermediate precision, trueness and accuracy, was obtained. Real patient plasma samples were then successfully analysed.
Sujets
PID Serval
serval:BIB_A9702E4751B4
PMID
Date de création
2009-10-01T14:52:58.919Z
Date de création dans IRIS
2025-05-20T23:14:44Z