Titre
The inhibition of fat cell proliferation by n-3 fatty acids in dietary obese mice.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Hensler, M.
Auteure/Auteur
Bardova, K.
Auteure/Auteur
Jilkova, Z.M.
Auteure/Auteur
Wahli, W.
Auteure/Auteur
Meztger, D.
Auteure/Auteur
Chambon, P.
Auteure/Auteur
Kopecky, J.
Auteure/Auteur
Flachs, P.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1476-511X
Statut éditorial
Publié
Date de publication
2011
Volume
10
Numéro
1
Première page
128
Langue
anglais
Résumé
ABSTRACT: BACKGROUND: Long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFA) of marine origin exert multiple beneficial effects on health. Our previous study in mice showed that reduction of adiposity by LC n-3 PUFA was associated with both, a shift in adipose tissue metabolism and a decrease in tissue cellularity. The aim of this study was to further characterize the effects of LC n-3 PUFA on fat cell proliferation and differentiation in obese mice. METHODS: A model of inducible and reversible lipoatrophy (aP2-Cre-ERT2 PPARgammaL2/L2 mice) was used, in which the death of mature adipocytes could be achieved by a selective ablation of peroxisome proliferator-activated receptor gamma in response to i.p. injection of tamoxifen. Before the injection, obesity was induced in male mice by 8-week-feeding a corn oil-based high-fat diet (cHF) and, subsequently, mice were randomly assigned (day 0) to one of the following groups: (i) mice injected by corn-oil-vehicle only, i.e."control" mice, and fed cHF; (ii) mice injected by tamoxifen in corn oil, i.e. "mutant" mice, fed cHF; (iii) control mice fed cHF diet with 15% of dietary lipids replaced by LC n-3 PUFA concentrate (cHF+F); and (iv) mutant mice fed cHF+F. Blood and tissue samples were collected at days 14 and 42. RESULTS: Mutant mice achieved a maximum weight loss within 10 days post-injection, followed by a compensatory body weight gain, which was significantly faster in the cHF as compared with the cHF+F mutant mice. Also in control mice, body weight gain was depressed in response to dietary LC n-3 PUFA. At day 42, body weights in all groups stabilized, with no significant differences in adipocyte size between the groups, although body weight and adiposity was lower in the cHF+F as compared with the cHF mice, with a stronger effect in the mutant than in control mice. Gene expression analysis documented depression of adipocyte maturation during the reconstitution of adipose tissue in the cHF+F mutant mice. CONCLUSION: Dietary LC n-3 PUFA could reduce both hypertrophy and hyperplasia of fat cells in vivo. Results are in agreement with the involvement of fat cell turnover in control of adiposity.
Sujets
PID Serval
serval:BIB_C2A61BCE5407
PMID
Open Access
Oui
Date de création
2011-08-15T08:11:20.327Z
Date de création dans IRIS
2025-05-20T21:50:28Z
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Nom
BIB_C2A61BCE5407.P001.pdf
Version du manuscrit
preprint
Taille
318.71 KB
Format
Adobe PDF
PID Serval
serval:BIB_C2A61BCE5407.P001
URN
urn:nbn:ch:serval-BIB_C2A61BCE54075
Somme de contrôle
(MD5):b7424b4a21b677e4f91c6e48d7ef1cc0