Titre
Endothelial Colony-Forming Cells Dysfunctions Are Associated with Arterial Hypertension in a Rat Model of Intrauterine Growth Restriction.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Simoncini, S.
Auteure/Auteur
Coppola, H.
Auteure/Auteur
Rocca, A.
Auteure/Auteur
Bachmann, I.
Auteure/Auteur
Guillot, E.
Auteure/Auteur
Zippo, L.
Auteure/Auteur
Dignat-George, F.
Auteure/Auteur
Sabatier, F.
Auteure/Auteur
Bedel, R.
Auteure/Auteur
Wilson, A.
Auteure/Auteur
Rosenblatt-Velin, N.
Auteure/Auteur
Armengaud, J.B.
Auteure/Auteur
Menétrey, S.
Auteure/Auteur
Peyter, A.C.
Co-dernière auteure/Co-dernier auteur
Simeoni, U.
Co-dernière auteure/Co-dernier auteur
Yzydorczyk, C.
Co-dernière auteure/Co-dernier auteur
Liens vers les unités
ISSN
1422-0067
Statut éditorial
Publié
Date de publication
2021-09-21
Volume
22
Numéro
18
Première page
10159
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
Infants born after intrauterine growth restriction (IUGR) are at risk of developing arterial hypertension at adulthood. The endothelium plays a major role in the pathogenesis of hypertension. Endothelial colony-forming cells (ECFCs), critical circulating components of the endothelium, are involved in vasculo-and angiogenesis and in endothelium repair. We previously described impaired functionality of ECFCs in cord blood of low-birth-weight newborns. However, whether early ECFC alterations persist thereafter and could be associated with hypertension in individuals born after IUGR remains unknown. A rat model of IUGR was induced by a maternal low-protein diet during gestation versus a control (CTRL) diet. In six-month-old offspring, only IUGR males have increased systolic blood pressure (tail-cuff plethysmography) and microvascular rarefaction (immunofluorescence). ECFCs isolated from bone marrow of IUGR versus CTRL males displayed a decreased proportion of CD31+ versus CD146+ staining on CD45- cells, CD34 expression (flow cytometry, immunofluorescence), reduced proliferation (BrdU incorporation), and an impaired capacity to form capillary-like structures (Matrigel test), associated with an impaired angiogenic profile (immunofluorescence). These dysfunctions were associated with oxidative stress (increased superoxide anion levels (fluorescent dye), decreased superoxide dismutase protein expression, increased DNA damage (immunofluorescence), and stress-induced premature senescence (SIPS; increased beta-galactosidase activity, increased p16 <sup>INK4a</sup> , and decreased sirtuin-1 protein expression). This study demonstrated an impaired functionality of ECFCs at adulthood associated with arterial hypertension in individuals born after IUGR.
Sujets
PID Serval
serval:BIB_76C775F89D99
PMID
Open Access
Oui
Date de création
2021-10-04T08:59:14.744Z
Date de création dans IRIS
2025-06-03T12:19:56Z
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Nom
34576323_BIB_76C775F89D99.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by/4.0
Taille
10.33 MB
Format
Adobe PDF
PID Serval
serval:BIB_76C775F89D99.P001
URN
urn:nbn:ch:serval-BIB_76C775F89D993
Somme de contrôle
(MD5):313a7be71727d72c2ced6338179b65f0