Titre
Inhibitor-conjugated harmonic nanoparticles targeting fibroblast activation protein.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
De Matos, R.
Auteure/Auteur
Vuilleumier, J.
Auteure/Auteur
Mas, C.
Auteure/Auteur
Constant, S.
Auteure/Auteur
Staedler, D.
Auteure/Auteur
Gerber-Lemaire, S.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
2046-2069
Statut éditorial
Publié
Date de publication
2019-10-01
Volume
9
Numéro
54
Première page
31659
Dernière page/numéro d’article
31669
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
The recent progress in the engineering of nanosized inorganic materials presenting tailored physical properties and reactive surface for post-functionalization has opened promising avenues for the use of nanoparticles (NPs) in diagnosis and therapeutic intervention. Surface decoration of metal oxide NPs with ligands modulating circulation time, cellular uptake, affinity and extravasation through active targeting led to efficient cancer specific bioimaging probes. The most relevant cancer biomarkers studied so far include surface and transmembrane cancer cell receptors. More recently, tumor microenvironments and more specifically the fibroblastic element of the tumor stroma have emerged as a valuable target for diagnosis and treatment of several types of cancers. In this study, a low molecular weight ligand targeting fibroblast activation protein α (FAP), which is specifically expressed by activated fibroblasts of the tumor stroma, was synthesized. This ligand demonstrated nanomolar inhibition of FAP with high selectivity with respect to prolyl oligopeptidase (PREP) and dipeptidyl peptidase (DPP) IV, as well as good biocompatibility toward a human lung tissue model. Bismuth ferrite (BFO) harmonic nanoparticles (HNPs) conjugated to this ligand showed target-specific association to FAP as demonstrated by reverse ELISA-type assay using Human Fibroblast Activation Protein alpha/FAP Alexa Fluor® 594-conjugated Antibody and multiphoton multispectral microscopy experiments. These functionalized HNPs may provide new nanocarriers to explore the role of FAP in tumorigenesis and to target the fibroblastic component of the tumor microenvironment.
PID Serval
serval:BIB_662D30113F1F
PMID
Open Access
Oui
Date de création
2019-10-06T12:29:02.155Z
Date de création dans IRIS
2025-05-21T01:28:37Z
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Nom
35527932_BIB_662D30113F1F.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by-nc/4.0
Taille
750.1 KB
Format
Adobe PDF
PID Serval
serval:BIB_662D30113F1F.P001
URN
urn:nbn:ch:serval-BIB_662D30113F1F9
Somme de contrôle
(MD5):a535bc917d5c5aa99b8c8022cd1a4090