Titre
Purification of potato leaf plasma membrane protein PP34, a protein phosphorylated in response to oligogalacturonide signals for defense and development.
Type
article
Institution
Externe
Périodique
Auteur(s)
Jacinto, T.
Auteure/Auteur
Farmer, E.E.
Auteure/Auteur
Ryan, C.A.
Auteure/Auteur
Liens vers les personnes
ISSN
1532-2548
Statut éditorial
Publié
Date de publication
1993
Volume
103
Numéro
4
Première page
1393
Dernière page/numéro d’article
1397
Langue
anglais
Résumé
A potato (Solanum tuberosum L.) plasma membrane protein called pp34, the only known example of a plasma membrane protein that is phosphorylated specifically in response to defined Oligogalacturonide signals in plants, has been purified to apparent homogeneity. Polyclonal antibodies raised in rabbits against the purified pp34 protein immunoprecipitated a single thiophosphorylated protein species from potato plasma membranes, as analyzed by two-dimensional denaturing electrophoresis and fluorography. The pp34 antibodies also recognized a single protein in tomato (Lycopersicon esculentum L.) membranes that is thiophosphorylated in response to Oligogalacturonide elicitors, as demonstrated by western blotting and specific immunoprecipitation. These experiments confirm the identity of the tomato membrane protein as a pp34 homolog and establish the high monospecificity of the pp34 antibodies. This will permit further investigation of the role of protein phosphorylation in oligouronide signaling for defensive genes in potato and tomato plants.
PID Serval
serval:BIB_5F2788C2B4BF
PMID
Open Access
Oui
Date de création
2008-01-24T19:05:37.937Z
Date de création dans IRIS
2025-05-20T17:45:03Z