Titre
Chromatin immunoprecipitation improvements for the processing of small frozen pieces of adipose tissue.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Castellano-Castillo, D.
Auteure/Auteur
Denechaud, P.D.
Auteure/Auteur
Moreno-Indias, I.
Auteure/Auteur
Tinahones, F.
Auteure/Auteur
Fajas, L.
Auteure/Auteur
Queipo-Ortuño, M.I.
Auteure/Auteur
Cardona, F.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1932-6203
Statut éditorial
Publié
Date de publication
2018
Volume
13
Numéro
2
Première page
e0192314
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Publication Status: epublish
Résumé
Chromatin immunoprecipitation (ChIP) has gained importance to identify links between the genome and the proteome. Adipose tissue has emerged as an active tissue, which secretes a wide range of molecules that have been related to metabolic and obesity-related disorders, such as diabetes, cardiovascular failure, metabolic syndrome, or cancer. In turn, epigenetics has raised the importance in discerning the possible relationship between metabolic disorders, lifestyle and environment. However, ChIP application in human adipose tissue is limited by several factors, such as sample size, frozen sample availability, high lipid content and cellular composition of the tissue. Here, we optimize the standard protocol of ChIP for small pieces of frozen human adipose tissue. In addition, we test ChIP for the histone mark H3K4m3, which is related to active promoters, and validate the performance of the ChIP by analyzing gene promoters for factors usually studied in adipose tissue using qPCR. Our improvements result in a higher performance in chromatin shearing and DNA recovery of adipocytes from the tissue, which may be useful for ChIP-qPCR or ChIP-seq analysis.
PID Serval
serval:BIB_5E840542134A
PMID
Open Access
Oui
Date de création
2018-02-15T16:25:04.073Z
Date de création dans IRIS
2025-05-20T20:25:16Z
Fichier(s)![Vignette d'image]()
En cours de chargement...
Nom
journal.pone.0192314.pdf
Version du manuscrit
published
Taille
2.87 MB
Format
Adobe PDF
PID Serval
serval:BIB_5E840542134A.P001
URN
urn:nbn:ch:serval-BIB_5E840542134A5
Somme de contrôle
(MD5):c8839e194ad739f01a4ec6eaaadca191