Titre
Functional significance of thermosensitive transient receptor potential melastatin channel 8 (TRPM8) expression in immortalized human corneal endothelial cells.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Mergler, S.
Auteure/Auteur
Mertens, C.
Auteure/Auteur
Valtink, M.
Auteure/Auteur
Reinach, P.S.
Auteure/Auteur
Székely, V.C.
Auteure/Auteur
Slavi, N.
Auteure/Auteur
Garreis, F.
Auteure/Auteur
Abdelmessih, S.
Auteure/Auteur
Türker, E.
Auteure/Auteur
Fels, G.
Auteure/Auteur
Pleyer, U.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1096-0007
Statut éditorial
Publié
Date de publication
2013
Volume
116
Première page
337
Dernière page/numéro d’article
349
Langue
anglais
Résumé
Human corneal endothelial cells (HCEC) maintain appropriate tissue hydration and transparency by eliciting net ion transport coupled to fluid egress from the stroma into the anterior chamber. Such activity offsets tissue swelling caused by stromal imbibition of fluid. As corneal endothelial (HCE) transport function is modulated by temperature changes, we probed for thermosensitive transient receptor potential melastatin 8 (TRPM8) functional activity in immortalized human corneal endothelial cells (HCEC-12) and freshly isolated human corneal endothelial cells (HCEC) as a control. This channel is either activated upon lowering to 28 °C or by menthol, eucalyptol and icilin. RT-PCR and quantitative real-time PCR (qPCR) verified TRPM8 gene expression. Ca(2+) transients induced by either menthol (500 μmol/l), eucalyptol (3 mmol/l), or icilin (2-60 μmol/l) were identified using cell fluorescence imaging. The TRP channel blocker lanthanum III chloride (La(3+), 100 μmol/l) as well as the TRPM8 blockers BCTC (10 μmol/l) and capsazepine (CPZ, 10 μmol/l) suppressed icilin-induced Ca(2+) increases. In and outward currents induced by application of menthol (500 μmol/l) or icilin (50 μmol/l) were detected using the planar patch-clamp technique. A thermal transition from room temperature to ≈ 18 °C led to Ca(2+) increases that were inhibited by a TRPM8 blocker BCTC (10 μmol/l). Other thermosensitive TRP pathways whose heterogeneous Ca(2+) response patterns are suggestive of other Ca(2+) handling pathways were also detected upon strong cooling (≈10 °C). Taken together, functional TRPM8 expression in HCEC-12 and freshly dissociated HCEC suggests that HCE function can adapt to thermal variations through activation of this channel subtype.
PID Serval
serval:BIB_FD9CAC0000C0
PMID
Date de création
2014-08-28T08:50:49.528Z
Date de création dans IRIS
2025-05-21T06:49:04Z