Titre
Reduced expression of Hugl-1, the human homologue of Drosophila tumour suppressor gene lgl, contributes to progression of colorectal cancer
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Schimanski, C. C.
Auteure/Auteur
Schmitz, G.
Auteure/Auteur
Kashyap, A.
Auteure/Auteur
Bosserhoff, A. K.
Auteure/Auteur
Bataille, F.
Auteure/Auteur
Schafer, S. C.
Auteure/Auteur
Lehr, H. A.
Auteure/Auteur
Berger, M. R.
Auteure/Auteur
Galle, P. R.
Auteure/Auteur
Strand, S.
Auteure/Auteur
Strand, D.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0950-9232
Statut éditorial
Publié
Date de publication
2005
Volume
24
Numéro
19
Première page
3100
Dernière page/numéro d’article
3109
Notes
PT - Journal Article PT - Research Support, Non-U.S. Gov't
Résumé
The human gene, human giant larvae (Hugl-1/Llg1/Lgl1) has significant homology to the Drosophila tumour suppressor gene lethal(2)giant larvae (lgl). The lgl gene codes for a cortical cytoskeleton protein, Lgl, that binds Myosin II and is involved in maintaining cell polarity and epithelial integrity. The human protein, Hugl-1 contains several conserved functional domains found in Lgl, suggesting that these proteins may have closely related functions. Whether loss of Hugl expression plays a role in human tumorigenesis has so far not been extensively investigated. Thus, we evaluated tumour tissues from 94 patients undergoing surgery for colorectal cancer (CRC) for loss of Hugl-1 transcription and compared our findings with the clinical data from each of these patients. We found that Hugl-1 was lost in 75% of tumour samples and these losses were associated with advanced stage and particularly with lymph node metastases. Reduced Hugl-1 expression during the adenoma-carcinoma sequence occurring as early as in colorectal adenomas was detected by both immunohistochemical and reverse transcription-polymerase chain reaction analysis. Functional assays with ecdysone-inducible cell lines revealed that Hugl-1 expression increased cell adhesion and decreased cell migration. Our studies thus indicate that downregulation of Hugl-1 contributes to CRC progression
Sujets
PID Serval
serval:BIB_8C1EAECC6C46
PMID
Date de création
2008-01-29T17:33:51.200Z
Date de création dans IRIS
2025-05-21T04:50:11Z