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  4. Exon junction complex subunits are required to splice Drosophila MAP kinase, a large heterochromatic gene.
 
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Titre

Exon junction complex subunits are required to splice Drosophila MAP kinase, a large heterochromatic gene.

Type
article
Institution
Externe
Périodique
Cell  
Auteur(s)
Roignant, J.Y.
Auteure/Auteur
Treisman, J.E.
Auteure/Auteur
Liens vers les personnes
Roignant, Jean-Yves  
ISSN
1097-4172
Statut éditorial
Publié
Date de publication
2010-10-15
Volume
143
Numéro
2
Première page
238
Dernière page/numéro d’article
250
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural
Publication Status: ppublish
Résumé
The exon junction complex (EJC) is assembled on spliced mRNAs upstream of exon-exon junctions and can regulate their subsequent translation, localization, or degradation. We isolated mutations in Drosophila mago nashi (mago), which encodes a core EJC subunit, based on their unexpectedly specific effects on photoreceptor differentiation. Loss of Mago prevents epidermal growth factor receptor signaling, due to a large reduction in MAPK mRNA levels. MAPK expression also requires the EJC subunits Y14 and eIF4AIII and EJC-associated splicing factors. Mago depletion does not affect the transcription or stability of MAPK mRNA but alters its splicing pattern. MAPK expression from an exogenous promoter requires Mago only when the template includes introns. MAPK is the primary functional target of mago in eye development; in cultured cells, Mago knockdown disproportionately affects other large genes located in heterochromatin. These data support a nuclear role for EJC components in splicing a specific subset of introns.
Sujets

Animals

Cell Differentiation

Drosophila Proteins/g...

Drosophila Proteins/m...

Drosophila melanogast...

Drosophila melanogast...

Drosophila melanogast...

ErbB Receptors/geneti...

Exons

Eye/embryology

Gene Knockdown Techni...

Heterochromatin/metab...

Humans

Mitogen-Activated Pro...

Nuclear Proteins/gene...

Nuclear Proteins/meta...

Photoreceptor Cells, ...

RNA Splicing

Signal Transduction

Wings, Animal/embryol...

PID Serval
serval:BIB_54231003ECAE
DOI
10.1016/j.cell.2010.09.036
PMID
20946982
WOS
000283052200013
Permalien
https://iris.unil.ch/handle/iris/112798
Open Access
Oui
Date de création
2019-10-28T11:58:12.860Z
Date de création dans IRIS
2025-05-20T19:30:30Z
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