Titre
Subtractive hybridization techniques to study cellular senescence.
Type
chapitre
Institution
Externe
Auteur(s)
Sternberg, M.B.
Auteure/Auteur
Gepstein, S.
Auteure/Auteur
Liens vers les personnes
Maison d’édition
Springer
Titre du livre ou conférence/colloque
Methods in molecular biology
Statut éditorial
Publié
Date de publication
2007
Volume
371
Chapitre
21
Première page
289
Dernière page/numéro d’article
305
Peer-reviewed
Oui
Langue
anglais
Résumé
Molecular and cellular processes related to the senescence syndrome are determined by programs of differential gene expression. Subtractive cDNA hybridization is a powerful approach to identify and isolate differentially expressed genes in various systems. A highly effective method, termed suppression subtractive hybridization (SSH), has been applied for the generation of subtracted cDNA library of senescing leaves. The method consists of two main stages, the normalization step that equalizes the abundance of cDNAs within the target population and the subtraction step that eliminates the common sequences between the target and the driver populations. The successful generation of library containing high numbered of rare and abundant cDNA clones in senescing plant cells proves the applicability of this method for global identification of differentially expressed genes during cellular senescence.
PID Serval
serval:BIB_7F949D70930E
PMID
Date de création
2019-07-26T15:42:40.698Z
Date de création dans IRIS
2025-05-21T02:07:01Z