Titre
Inference of chromosomal inversion dynamics from Pool-Seq data in natural and laboratory populations of Drosophila melanogaster
Type
article
Institution
Externe
Périodique
Auteur(s)
Kapun, Martin
Auteure/Auteur
van Schalkwyk, Hester
Auteure/Auteur
McAllister, Bryant
Auteure/Auteur
Flatt, Thomas
Auteure/Auteur
Schlötterer, Christian
Auteure/Auteur
Liens vers les personnes
Statut éditorial
Publié
Date de publication
2014
Volume
23
Numéro
7
Première page
1813
Dernière page/numéro d’article
1827
Langue
anglais
Résumé
Sequencing of pools of individuals (Pool-Seq) represents a reliable and cost-effective approach for estimating genome-wide SNP and transposable element insertion frequencies. However, Pool-Seq does not provide direct information on haplotypes so that, for example, obtaining inversion frequencies has not been possible until now. Here, we have developed a new set of diagnostic marker SNPs for seven cosmopolitan inversions in Drosophila melanogaster that can be used to infer inversion frequencies from Pool-Seq data. We applied our novel marker set to Pool-Seq data from an experi- mental evolution study and from North American and Australian latitudinal clines. In the experimental evolution data, we find evidence that positive selection has driven the frequencies of In(3R)C and In(3R)Mo to increase over time. In the clinal data, we confirm the existence of frequency clines for In(2L)t, In(3L)P and In(3R)Payne in both North America and Australia and detect a previously unknown latitudinal cline for In (3R)Mo in North America. The inversion markers developed here provide a versatile and robust tool for characterizing inversion frequencies and their dynamics in Pool- Seq data from diverse D. melanogaster populations.
PID Serval
serval:BIB_1D8B5CD9677A
PMID
Date de création
2014-04-08T19:43:19.753Z
Date de création dans IRIS
2025-05-20T17:22:04Z