Titre
The cytosolic phorboid receptor correlates with hormone dependency in six mammary carcinoma cell lines
Type
article
Institution
Externe
Auteur(s)
Costa, S. D.
Auteure/Auteur
Fabbro, D.
Auteure/Auteur
Regazzi, R.
Auteure/Auteur
Kung, W.
Auteure/Auteur
Eppenberger, U.
Auteure/Auteur
Liens vers les personnes
ISSN
0006-291X
Statut éditorial
Publié
Date de publication
1985-12
Volume
133
Numéro
2
Première page
814
Dernière page/numéro d’article
22
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec 17
Research Support, Non-U.S. Gov't --- Old month value: Dec 17
Résumé
Potent, structurally different tumor promoters inhibited growth of 6 human mammary carcinoma cell lines (ROOS et al, PNAS in press). This growth inhibition was investigated by measuring the phorboid receptor binding using [3H] PDBu (4 beta-phorbol 12, 13 dibutyrate). Specific, high affinity receptors were found in all six cell lines. [3H] PDBu binding affinities were higher in the cytosolic fractions than in the corresponding intact cells (K alpha = app. 1nM vs K alpha = app. 15nM). The hormone-independent cell lines (BT-20, HBL-100 and MDA-MB-231) exhibited significantly higher levels of cytosolic [3H] PDBu receptors than the hormone-dependent cells (MCF-7, T-47-D and ZR-75-1). The subcellular distribution of the [3H] PDBu binding correlated well with the distribution of the protein kinase C activity (r = 0.95).
Sujets
PID Serval
serval:BIB_76623B0C821B
PMID
Date de création
2008-01-24T13:30:08.089Z
Date de création dans IRIS
2025-05-21T04:11:20Z