Titre
Chronic bronchial allergic inflammation increases alveolar liquid clearance by TNF-alpha -dependent mechanism.
Type
article
Institution
Externe
Auteur(s)
Tillie-Leblond, I.
Auteure/Auteur
Guery, B.P.
Auteure/Auteur
Janin, A.
Auteure/Auteur
Leberre, R.
Auteure/Auteur
Just, N.
Auteure/Auteur
Pittet, J.F.
Auteure/Auteur
Tonnel, A.B.
Auteure/Auteur
Gosset, P.
Auteure/Auteur
Liens vers les personnes
ISSN
1040-0605
Statut éditorial
Publié
Date de publication
2002-12
Volume
283
Numéro
6
Première page
L1303
Dernière page/numéro d’article
9
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Bronchial inflammation in allergic asthma is associated with active exudation from the bronchial tree into the interstitial space of both mucosa and submucosa. The aim of this study was to evaluate epithelial and endothelial permeability as well as alveolar fluid movement in a model of chronic allergic inflammation in Brown-Norway rats sensitized and challenged with ovalbumin (OA). Control groups were challenged with saline solution (C), and rats were immunized by OA but not challenged (Se). Lung sections showed a marked inflammatory infiltrate associated with perivascular and peribronchiolar edema in OA. To measure alveolar liquid clearance, a 5% bovine albumin solution with 1 microCi of (125)I-labeled human albumin was instilled into the air spaces. Alveolar-capillary barrier permeability was evaluated by intravascular injection of 1 microCi of (131)I-labeled albumin. Endothelial permeability was significantly increased in OA, from 0.08 +/- 0.01 in the C group to 0.19 +/- 0.03 in OA group (P < 0.05). Final-to-initial protein ratio was also statistically higher in OA (1.6 +/- 0.05) compared with C (1.38 +/- 0.03, P = 0.01) and Se groups (1.42 +/- 0.03, P = 0.04). Administration of anti-tumor necrosis factor-alpha antibodies within the instillate significantly decreased this ratio (1.32 +/- 0.08, P = 0.003 vs. OA). To conclude, we demonstrated a tumor necrosis factor-alpha-dependent increase in alveolar fluid movement in a model of severe bronchial allergic inflammation associated with endothelial and epithelial leakage.
Sujets
PID Serval
serval:BIB_AA1344928BF7
PMID
Date de création
2021-04-29T08:59:10.751Z
Date de création dans IRIS
2025-05-21T02:20:12Z