Titre
Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Telenti, A.
Auteure/Auteur
Marchesi, F.
Auteure/Auteur
Balz, M.
Auteure/Auteur
Bally, F.
Auteure/Auteur
Bottger, E. C.
Auteure/Auteur
Bodmer, T.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0095-1137
Statut éditorial
Publié
Date de publication
1993-02
Volume
31
Numéro
2
Première page
175
Dernière page/numéro d’article
8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Research Support, Non-U.S. Gov't --- Old month value: Feb
Résumé
A method for the rapid identification of mycobacteria to the species level was developed on the basis of evaluation by the polymerase chain reaction (PCR) of the gene encoding for the 65-kDa protein. The method involves restriction enzyme analysis of PCR products obtained with primers common to all mycobacteria. Using two restriction enzymes, BstEII and HaeIII, medically relevant and other frequent laboratory isolates were differentiated to the species or subspecies level by PCR-restriction enzyme pattern analysis. PCR-restriction enzyme pattern analysis was performed on isolates (n = 330) from solid and fluid culture media, including BACTEC, or from frozen and lyophilized stocks. The procedure does not involve hybridization steps or the use of radioactivity and can be completed within 1 working day.
Sujets
PID Serval
serval:BIB_97B6765E6192
PMID
Date de création
2008-01-25T13:46:23.240Z
Date de création dans IRIS
2025-05-21T04:57:01Z