Titre
GC-MS Based Metabolomics and NMR Spectroscopy Investigation of Food Intake Biomarkers for Milk and Cheese in Serum of Healthy Humans.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Trimigno, A.
Auteure/Auteur
Münger, L.
Auteure/Auteur
Picone, G.
Auteure/Auteur
Freiburghaus, C.
Auteure/Auteur
Pimentel, G.
Auteure/Auteur
Vionnet, N.
Auteure/Auteur
Pralong, F.
Auteure/Auteur
Capozzi, F.
Auteure/Auteur
Badertscher, R.
Auteure/Auteur
Vergères, G.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
2218-1989
Statut éditorial
Publié
Date de publication
2018-03-23
Volume
8
Numéro
2
Première page
NA
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
The identification and validation of food intake biomarkers (FIBs) in human biofluids is a key objective for the evaluation of dietary intake. We report here the analysis of the GC-MS and 1H-NMR metabolomes of serum samples from a randomized cross-over study in 11 healthy volunteers having consumed isocaloric amounts of milk, cheese, and a soy drink as non-dairy alternative. Serum was collected at baseline, postprandially up to 6 h, and 24 h after consumption. A multivariate analysis of the untargeted serum metabolomes, combined with a targeted analysis of candidate FIBs previously reported in urine samples from the same study, identified galactitol, galactonate, and galactono-1,5-lactone (milk), 3-phenyllactic acid (cheese), and pinitol (soy drink) as candidate FIBs for these products. Serum metabolites not previously identified in the urine samples, e.g., 3-hydroxyisobutyrate after cheese intake, were detected. Finally, an analysis of the postprandial behavior of candidate FIBs, in particular the dairy fatty acids pentadecanoic acid and heptadecanoic acid, revealed specific kinetic patterns of relevance to their detection in future validation studies. Taken together, promising candidate FIBs for dairy intake appear to be lactose and metabolites thereof, for lactose-containing products, and microbial metabolites derived from amino acids, for fermented dairy products such as cheese.
PID Serval
serval:BIB_2B78DE188FA1
PMID
Open Access
Oui
Date de création
2018-03-29T18:22:41.402Z
Date de création dans IRIS
2025-05-20T14:23:40Z
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Nom
29570652.pdf
Version du manuscrit
published
Taille
26.89 MB
Format
Adobe PDF
PID Serval
serval:BIB_2B78DE188FA1.P001
URN
urn:nbn:ch:serval-BIB_2B78DE188FA17
Somme de contrôle
(MD5):e601db57e61546e241353cfcd5a71202