Titre
The CaMV 35S promoter has a weak expression activity in dark grown tissues of moss Physcomitrella patens.
Type
lettre à l'éditeur
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Plant Signaling and Behavior
Auteur(s)
Saidi, Y.
Auteure/Auteur
Schaefer, D.G.
Auteure/Auteur
Goloubinoff, P.
Auteure/Auteur
Zrÿd, J.P.
Auteure/Auteur
Finka, A.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1559-2324[electronic], 1559-2316[linking]
Statut éditorial
Publié
Date de publication
2009
Volume
4
Numéro
5
Première page
457
Dernière page/numéro d’article
459
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Comment ; Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
The constitutive Cauliflower Mosaic Virus 35S promoter (CaMV 35S) is widely used as a tool to express recombinant proteins in plants, but with different success. We previously showed that the expression of an F-actin marker, GFP-talin, in Physcomitrella patens using the CaMV 35S promoter failed to homogenously label moss tissues. Here, we show a significant diminution of the GFP fluorescence in dark grown old moss cells and complete lack of labelling in newly differentiated cells. Furthermore, we demonstrate that stable moss lines harbouring a resistance cassette driven by the CaMV 35S are unable to grow in darkness in the presence of the antibiotic. In contrast to the CaMV 35S, the heat inducible promoter, hsp17.3B showed uniform expression pattern in all cells and tissues following a mild heat shock.
PID Serval
serval:BIB_644E639A8682
PMID
Open Access
Oui
Date de création
2009-11-23T12:18:07.734Z
Date de création dans IRIS
2025-05-20T21:49:04Z