Titre
Renin and angiotensinogen expression and functions in growth and apoptosis of human glioblastoma.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Juillerat-Jeanneret, L.
Auteure/Auteur
Celerier, J.
Auteure/Auteur
Chapuis Bernasconi, C.
Auteure/Auteur
Nguyen, G.
Auteure/Auteur
Wostl, W.
Auteure/Auteur
Maerki, H.P.
Auteure/Auteur
Janzer, R.C.
Auteure/Auteur
Corvol, P.
Auteure/Auteur
Gasc, J.M.
Auteure/Auteur
Liens vers les personnes
rjanzer
Liens vers les unités
ISSN
0007-0920
Statut éditorial
Publié
Date de publication
2004-03-08
Volume
90
Numéro
5
Première page
1059
Dernière page/numéro d’article
1068
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
The expression and function in growth and apoptosis of the renin-angiotensin system (RAS) was evaluated in human glioblastoma. Renin and angiotensinogen (AGT) mRNAs and proteins were found by in situ hybridisation and immunohistochemistry in glioblastoma cells. Angiotensinogen was present in glioblastoma cystic fluids. Thus, human glioblastoma cells produce renin and AGT and secrete AGT. Human glioblastoma and glioblastoma cells expressed renin, AGT, renin receptor, AT(2) and/or AT(1) mRNAs and proteins determined by RT-PCR and/or Western blotting, respectively. The function of the RAS in glioblastoma was studied using human glioblastoma cells in culture. Angiotensinogen, des(Ang I)AGT, tetradecapaptide renin substrate (AGT1-14), Ang I, Ang II or Ang III, added to glioblastoma cells in culture, did not modulate their proliferation, survival or death. Angiotensin-converting enzyme inhibitors did not diminish glioblastoma cell proliferation. However, the addition of selective synthetic renin inhibitors to glioblastoma cells decreased DNA synthesis and viable tumour cell number, and induced apoptosis. This effect was not counterbalanced by concomitant addition of Ang II. In conclusion, the complete RAS is expressed by human glioblastomas and glioblastoma cells in culture. Inhibition of renin in glioblastoma cells may be a potential approach to control glioblastoma cell proliferation and survival, and glioblastoma progression in combination therapy.
Sujets
PID Serval
serval:BIB_6C9AF4F5BC4B
PMID
Open Access
Oui
Date de création
2008-01-29T17:34:42.172Z
Date de création dans IRIS
2025-05-20T23:58:46Z
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Nom
BIB_6C9AF4F5BC4B.P001.pdf
Version du manuscrit
published
Taille
419.45 KB
Format
Adobe PDF
PID Serval
serval:BIB_6C9AF4F5BC4B.P001
URN
urn:nbn:ch:serval-BIB_6C9AF4F5BC4B4
Somme de contrôle
(MD5):ffade35840525fda183d011c93d52b44