Titre
Morphological and physiological species-dependent characteristics of the rodent Grueneberg ganglion.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Brechbühl, J.
Auteure/Auteur
Klaey, M.
Auteure/Auteur
Moine, F.
Auteure/Auteur
Bovay, E.
Auteure/Auteur
Hurni, N.
Auteure/Auteur
Nenniger-Tosato, M.
Auteure/Auteur
Broillet, M.C.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1662-5129
Statut éditorial
Publié
Date de publication
2014
Volume
8
Première page
87
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article Publication Status: epublish
Résumé
In the mouse, the Grueneberg ganglion (GG) is an olfactory subsystem implicated both in chemo- and thermo-sensing. It is specifically involved in the recognition of volatile danger cues such as alarm pheromones and structurally-related predator scents. No evidence for these GG sensory functions has been reported yet in other rodent species. In this study, we used a combination of histological and physiological techniques to verify the presence of a GG and investigate its function in the rat, hamster, and gerbil comparing with the mouse. By scanning electron microscopy (SEM) and transmitted electron microscopy (TEM), we found isolated or groups of large GG cells of different shapes that in spite of their gross anatomical similarities, display important structural differences between species. We performed a comparative and morphological study focusing on the conserved olfactory features of these cells. We found fine ciliary processes, mostly wrapped in ensheating glial cells, in variable number of clusters deeply invaginated in the neuronal soma. Interestingly, the glial wrapping, the amount of microtubules and their distribution in the ciliary processes were different between rodents. Using immunohistochemistry, we were able to detect the expression of known GG proteins, such as the membrane guanylyl cyclase G and the cyclic nucleotide-gated channel A3. Both the expression and the subcellular localization of these signaling proteins were found to be species-dependent. Calcium imaging experiments on acute tissue slice preparations from rodent GG demonstrated that the chemo- and thermo-evoked neuronal responses were different between species. Thus, GG neurons from mice and rats displayed both chemo- and thermo-sensing, while hamsters and gerbils showed profound differences in their sensitivities. We suggest that the integrative comparison between the structural morphologies, the sensory properties, and the ethological contexts supports species-dependent GG features prompted by the environmental pressure.
PID Serval
serval:BIB_59927BBE2E01
PMID
Open Access
Oui
Date de création
2014-10-02T16:29:22.366Z
Date de création dans IRIS
2025-05-20T20:27:42Z
Fichier(s)![Vignette d'image]()
En cours de chargement...
Nom
BIB_59927BBE2E01.P001.pdf
Version du manuscrit
preprint
Taille
37.39 MB
Format
Adobe PDF
PID Serval
serval:BIB_59927BBE2E01.P001
URN
urn:nbn:ch:serval-BIB_59927BBE2E010
Somme de contrôle
(MD5):d0cf69974724416ee776dc96bed4b378