Titre
Detection of respiratory bacterial pathogens causing atypical pneumonia by multiplex Lightmix<sup>®</sup> RT-PCR.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Auteur(s)
Wagner, K.
Auteure/Auteur
Springer, B.
Auteure/Auteur
Imkamp, F.
Auteure/Auteur
Opota, O.
Auteure/Auteur
Greub, G.
Auteure/Auteur
Keller, P.M.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1618-0607
Statut éditorial
Publié
Date de publication
2018-04
Volume
308
Numéro
3
Première page
317
Dernière page/numéro d’article
323
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Pneumonia is a severe infectious disease. In addition to common viruses and bacterial pathogens (e.g. Streptococcus pneumoniae), fastidious respiratory pathogens like Chlamydia pneumoniae, Mycoplasma pneumoniae and Legionella spp. can cause severe atypical pneumonia. They do not respond to penicillin derivatives, which may cause failure of antibiotic empirical therapy. The same applies for infections with B. pertussis and B. parapertussis, the cause of pertussis disease, that may present atypically and need to be treated with macrolides. Moreover, these fastidious bacteria are difficult to identify by culture or serology, and therefore often remain undetected. Thus, rapid and accurate identification of bacterial pathogens causing atypical pneumonia is crucial. We performed a retrospective method evaluation study to evaluate the diagnostic performance of the new, commercially available Lightmix <sup>®</sup> multiplex RT-PCR assay that detects these fastidious bacterial pathogens causing atypical pneumonia. In this retrospective study, 368 clinical respiratory specimens, obtained from patients suffering from atypical pneumonia that have been tested negative for the presence of common agents of pneumonia by culture and viral PCR, were investigated. These clinical specimens have been previously characterized by singleplex RT-PCR assays in our diagnostic laboratory and were used to evaluate the diagnostic performance of the respiratory multiplex Lightmix <sup>®</sup> RT-PCR. The multiplex RT-PCR displayed a limit of detection between 5 and 10 DNA copies for different in-panel organisms and showed identical performance characteristics with respect to specificity and sensitivity as in-house singleplex RT-PCRs for pathogen detection. The Lightmix <sup>®</sup> multiplex RT-PCR assay represents a low-cost, time-saving and accurate diagnostic tool with high throughput potential. The time-to-result using an automated DNA extraction device for respiratory specimens followed by multiplex RT-PCR detection was below 4 h, which is expected to significantly improve diagnostics for atypical pneumonia-associated bacterial pathogens.
Sujets
PID Serval
serval:BIB_D7A3ACFF6749
PMID
Open Access
Oui
Date de création
2018-02-08T17:30:39.950Z
Date de création dans IRIS
2025-05-21T04:06:02Z
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Nom
1-s2.0-S1438422117304721-main.pdf
Version du manuscrit
published
Taille
588.07 KB
Format
Adobe PDF
PID Serval
serval:BIB_D7A3ACFF6749.P001
URN
urn:nbn:ch:serval-BIB_D7A3ACFF67490
Somme de contrôle
(MD5):6ce4bb1400aab4f7c7a75f895a1aa8cb