Titre

Genomic Study of RNA Polymerase II and III SNAP(c)-Bound Promoters Reveals a Gene Transcribed by Both Enzymes and a Broad Use of Common Activators.

Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
James Faresse, N.
Auteure/Auteur
Canella, D.
Auteure/Auteur
Praz, V.
Auteure/Auteur
Michaud, J.
Auteure/Auteur
Romascano, D.
Auteure/Auteur
Hernandez, N.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1553-7404
Statut éditorial
Publié
Date de publication
2012
Volume
8
Numéro
11
Première page
e1003028
Langue
anglais
Résumé
SNAP(c) is one of a few basal transcription factors used by both RNA polymerase (pol) II and pol III. To define the set of active SNAP(c)-dependent promoters in human cells, we have localized genome-wide four SNAP(c) subunits, GTF2B (TFIIB), BRF2, pol II, and pol III. Among some seventy loci occupied by SNAP(c) and other factors, including pol II snRNA genes, pol III genes with type 3 promoters, and a few un-annotated loci, most are primarily occupied by either pol II and GTF2B, or pol III and BRF2. A notable exception is the RPPH1 gene, which is occupied by significant amounts of both polymerases. We show that the large majority of SNAP(c)-dependent promoters recruit POU2F1 and/or ZNF143 on their enhancer region, and a subset also recruits GABP, a factor newly implicated in SNAP(c)-dependent transcription. These activators associate with pol II and III promoters in G1 slightly before the polymerase, and ZNF143 is required for efficient transcription initiation complex assembly. The results characterize a set of genes with unique properties and establish that polymerase specificity is not absolute in vivo.
PID Serval
serval:BIB_B581994FAB4A
DOI
PMID
WOS
Permalien
Open Access
Oui
Date de création
2012-12-20T15:47:55.610Z
Date de création dans IRIS
2025-05-21T04:50:07Z
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BIB_B581994FAB4A.P001.pdf

Version du manuscrit

preprint

Taille

1.99 MB

Format

Adobe PDF

PID Serval

serval:BIB_B581994FAB4A.P001

URN

urn:nbn:ch:serval-BIB_B581994FAB4A8

Somme de contrôle

(MD5):c7926844c24336f94450f691ede144f0